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Site-specific labelling of proteins

  • US 7,449,558 B2
  • Filed: 10/29/2004
  • Issued: 11/11/2008
  • Est. Priority Date: 10/30/2003
  • Status: Expired due to Fees
First Claim
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1. A method for labelling a protein in a cell, comprising introducing a detectable probe having a thioester group into a cell expressing a protein that is proteolytically processed to expose an N-terminal cysteine;

  • and washing the cell;

    wherein the probe is acetic acid 4-[(benzylsulfanylcarbonylmethyl-carbamoyl)-methyl]-2-oxo-2H-chromen-7-yl ester;

    N-benzylsulfanylcarbonylmethyl-6-(3,6-dihydroxy-3H-xanthen-9-yl)-isophthalamic acid;

    amino thioacetic acid S-benzyl ester of 5-carboxy-tetramethylrhodamine;

    amino thioacetic acid S-benzyl ester of acetoxynapthofluorescein;

    [5-(2-oxo-hexahydro-thieno[3,4-d]imidazol-6-yl)-pentanoylamino]-thioacetic acid S-benzyl ester;

    (4-benzoyl-benzoylamino)-thioacetic acid S-benzyl ester;

    or amino thioacetic acid S-benzyl ester of caged fluorescein, and is cell-permeable.

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