Intracorporeal substance measuring assembly

• US 7,450,980 B2
• Filed: 03/25/2005
• Issued: 11/11/2008
• Est. Priority Date: 03/31/2004
• Status: Active Grant

• Current Assignee: Terumo Kabushiki Kaisha
• Original Assignee: Terumo Kabushiki Kaisha
• Inventors: Kawanishi, Tetsuro
• Primary Examiner(s): Winakur; Eric F.
• Assistant Examiner(s): Berhanu; Etsub D
• Application Number:
  US11/089,329
• Publication Number:
  US 20050221277A1
• Time in Patent Office: 1,327 Days
• Field of Search: 600/310, 600/316, 600/317
• US Class Current: 600/317
• CPC Class Codes: A61B 5/14532 for measuring glucose, e.g. by tissue impedance measurement
• CPC Class Codes: A61B 5/1459 invasive, e.g. introduced into the body by a catheter A61B5/1464 takes precedence

Abstract

An intracorporeal substance measuring assembly to be provided in an embedded-type substance sensor for detecting and measuring an intercorporeal analyte includes: a detection layer containing at least one fluorescent indicator for generating fluorescence according to the concentration of the analyte; and an optical separation layer which is provided on the detection layer, is optically opaque, permits the analyte to penetrate therethrough, and prevents the penetration therethrough of at least one of living body substances possibly deteriorating the detection layer and/or obstructing the fluorescence.

Claims

1. An intracorporeal substance measuring assembly provided in an embedded type substance sensor for detecting and measuring an intracorporeal analyte, comprising:

a detection layer containing at least one fluorescent indicator containing phenyl boronic acid that generates fluorescence according to the concentration of said analyte; and

an optical separation layer on one side of said detection layer that is optically opaque and permits said analyte to penetrate therethrough;

wherein said analyte is a saccharide, and said fluorescent indicator is represented by formula (1);

2. The intracorporeal substance measuring assembly as set forth in claim 1, wherein Q is a fluorescent residual group containing anthracene.

3. The intracorporeal substance measuring assembly as set forth in claim 1, wherein said optical separation layer is comprised of an opaque substance and a base material for supporting said opaque substance.

4. The intracorporeal substance measuring assembly as set forth in claim 3, wherein said opaque substance is comprised of at least one substance selected from the group consisting of carbon black, fullerene, and carbon nanotube.

5. The intracorporeal substance measuring assembly as set forth in claim 1, wherein said optical separation layer is a polymeric porous film formed by a phase transition method.

6. The intracorporeal substance measuring assembly as set forth in claim 1, wherein said optical separation layer does not permit intracorporeal components that deteriorate said detection layer to penetrate therethrough.

7. The intracorporeal substance measuring assembly as set forth in claim 6, wherein said optical separation layer inactivates said intracorporeal components.

8. A method of producing an intracorporeal substance measuring assembly provided in an embedded type substance sensor for detecting and measuring an intracorporeal saccharide analyte the method comprising:

providing a detection layer containing at least one phenyl boronic acid-containing fluorescent indicator that fluoresces according to the concentration of said analyte; and

an optical separation layer disposed on one side of said detection layer that is optically opaque and permits said analyte to penetrate therethrough,

wherein said fluorescent indicator is represented by formula (1);

9. A method of producing an intracorporeal substance measuring assembly provided in an embedded type substance sensor for detecting and measuring an intracorporeal saccharide analyte the method comprising:

providing a detection layer containing at least one phenyl boronic acid-containing fluorescent indicator that fluoresces according to the concentration of said analyte; and

an optical separation layer disposed on one side of said detection layer that is optically opaque, and permits said analyte to penetrate therethrough,

wherein said fluorescent indicator is represented by formula (1);

10. An embedded type substance sensor comprising:

an intracorporeal substance measuring assembly provided in an embedded type substance sensor for detecting and measuring an intracorporeal saccharide analyte including;

a detection layer containing at least one phenyl boronic acid-containing fluorescent indicator that fluoresces according to the concentration of said analyte, wherein said fluorescent indicator is represented by formula (1);

11. The embedded type substance sensor as set forth in claim 10 wherein the optical layer side of said intracorporeal substance measuring assembly is configured to be in contact with a living body.