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Direct differentiation method for making cardiomyocytes from human embryonic stem cells

  • US 7,452,718 B2
  • Filed: 03/21/2005
  • Issued: 11/18/2008
  • Est. Priority Date: 03/26/2004
  • Status: Active Grant
First Claim
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1. A direct differentiation method of obtaining cardiomyocyte lineage cells from primate pluripotent stem (pPS) cells, comprising in the following order:

  • a) obtaining undifferentiated pPS cells;

    b) plating the undifferentiated pPS cells without forming embryoid bodies directly onto a solid surface comprising a substrate to which cardiomyocyte lineage cells adhere without forming embryoid bodies;

    c) permitting the plated pPS cells to adhere onto the substrate;

    d) culturing the adhered cells in the absence of serum or feeder cells, but in the presence of activin and a bone morphogenic protein to produce cardiomyocyte lineage cells;

    e) harvesting the cardiomyocyte lineage cells from the culture.

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