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Medium for growing human embryonic stem cells

  • US 7,455,983 B2
  • Filed: 09/24/2004
  • Issued: 11/25/2008
  • Est. Priority Date: 01/11/2000
  • Status: Expired due to Term
First Claim
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1. A method of evaluating a media for culturing human embryonic stem (hES) cells in vitro to determine if they proliferate without differentiating, comprising culturing hES cells on an extracellular matrix and in a medium comprising:

  • an isotonic buffer;

    a protein nutrient, comprising serum, serum replacement, albumin, or essential and non-essential amino acids;

    lipids, fatty acids, or cholesterol, either as artificial additives or as the HDL or LDL extract of serum;

    added fibroblast growth factor at a concentration of 40 ng/mL; and

    added Flt-3 ligand at a concentration of 15 ng/ml; and

    culturing hES cells in the medium through at least four passages; and

    determining if the hES cells cultured in the medium have differentiated.

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