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Sequencing of surface immobilized polymers utilizing microfluorescence detection

  • US 7,459,275 B2
  • Filed: 06/02/2006
  • Issued: 12/02/2008
  • Est. Priority Date: 12/06/1990
  • Status: Expired due to Fees
First Claim
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1. A method for analyzing a target nucleic acid comprising:

  • (a) providing a nucleic acid array, said array comprising at least two different target template nucleic acids attached to a support in positionally distinct regions at a density of at least 1000 regions per cm2;

    (b) contacting said nucleic acid array with at least one nucleotide comprising a removable label;

    (c) detecting the label to determine the addition of the nucleotide to an oligonucleotide hybridized to one of the template nucleic acids;

    (d) removing the label; and

    (e) repeating steps (b) and (c).

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