Molecularly-imprinted chemical detection device and method

• US 7,459,316 B2
• Filed: 07/08/2003
• Issued: 12/02/2008
• Est. Priority Date: 02/24/2003
• Status: Expired due to Fees

• Current Assignee: National Research Council Canada
• Original Assignee: National Research Council Canada
• Inventors: Bensebaa, Farid, Py, Christophe, Faid, Karim
• Primary Examiner(s): Warden; Jill
• Assistant Examiner(s): LEVKOVICH, NATALIA A
• Application Number:
  US10/614,158
• Publication Number:
  US 20080268550A1
• Time in Patent Office: 1,974 Days
• Field of Search: 435/4, 436/518
• US Class Current: 436/518
• CPC Class Codes: G01N 33/54366 Apparatus specially adapted for solid-phase testing

Abstract

A novel method of molecular imprinting is described. Using a modified soft lithography technique, a molecularly-imprinted chemical detection device comprising at least one molecularly-imprinted polymer capable of detecting at least one chemical target is produced. The device can be used in the field for in situ detection and quantification of chemical targets using standard surface analytical techniques.

Claims

1. A method of producing a molecularly-imprinted chemical detection device comprising the steps of:

a) modifying the surface of a solid support through the attachment of functional groups;

b) reacting the solid support with a derivatized molecular target to form a molecular target bound solid support;

c) reacting the molecular target bound solid support with at least one guest molecule thus forming a solid support stamp having a binding cavity around the molecular target, the binding cavity having one or more functional groups which allow attachment of the binding cavity to a solid substrate;

d) selecting the solid substrate for facilitating attachment of the binding cavity on the solid support stamp to the solid substrate;

e) applying the solid support stamp to a surface of the solid substrate to attach the binding cavity to the solid substrate; and

f) removing the molecular target with the solid support to produce a molecularly-imprinted polymer on the surface of the solid substrate.

2. The method of claim 1 wherein the at least one guest molecule is at least one derivatized functional monomer forming at least one functional monomer recognition element around the molecular target.

3. The method of claim 2 wherein at least two functional monomer recognition elements are polymerized the form the binding cavity around the molecular target.

4. The method of claim 1 wherein the guest molecule is at least one derivatized functional polymer.

5. The method of claim 1 wherein a silicon wafer solid support is modified to facilitate the attachment in step (a).

6. The method of claim 1 wherein the surface of the solid support is modified with amino-siloxane.

7. The method of claim 1 wherein the solid substrate is a coated slide.

8. The method of claim 7 wherein the coated slide is a coated glass slide.

9. The method of claim 7 wherein the coated slide is coated with one of gold and silicon dioxide.

10. The method of claim 1 wherein a plurality of binding cavities are attached to the solid substrate producing a plurality of molecularly-imprinted polymers.

11. The method of claim 10 wherein at least two different binding cavities are attached to the solid substrate producing at least two different molecularly-imprinted polymers segregated into separate areas.

12. The method of claim 1 wherein the solid support stamp is applied to a surface of a solid substrate having an area of about 1 to 2 cm².

13. A method of producing a molecularly-imprinted chemical detection device using a solid support stamp produced from a molecular target bound solid support, having a binding cavity around the molecular target, the binding cavity having one or more functional groups which allow attachment of the binding cavity to a solid substrate, comprising the steps of:

a) selecting the solid substrate for facilitating attachment of the binding cavity on the solid support stamp to the solid substrate;

b) applying the solid support stamp to the surface of the solid substrate to attach the binding cavity to the solid substrate; and

c) removing the molecular target bound solid support to produce a molecularly-imprinted polymer on the surface of the solid substrate.

14. The method of claim 13 wherein the solid support stamp is applied to a surface of a coated slide in step (b).

15. The method of claim 14 wherein the coated slide is a coated glass slide.

16. The method of claim 14 wherein the coated slide is coated in one of gold and silicon dioxide.

17. The method of claim 13 wherein a plurality of binding cavities are attached to the solid substrate producing a plurality of molecularly-imprinted polymers.

18. The method of claim 13 wherein at least two different binding cavities are attached to the solid substrate producing at least two different molecularly-imprinted polymers segregated into separate areas.