Subcutaneous glucose electrode
First Claim
1. A glucose or lactate sensor comprising:
- one or more working electrodes; and
an analyte-responsive sensing layer associated with and in electrical contact with the one or more working electrodes, the sensing layer comprising a glucose-specific or lactate-specific enzyme crosslinked with a polymer selected from the group consisting of poly(vinylimidazole), poly(vinyl pyridine), and poly(acrylamide co-I-vinylimidazole), wherein at least a portion of the sensor is adapted to be subcutaneously positioned in vivo in a subject.
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Abstract
A small diameter flexible electrode designed for subcutaneous in vivo amperometric monitoring of glucose is described. The electrode is designed to allow “one-point” in vivo calibration, i.e., to have zero output current at zero glucose concentration, even in the presence of other electroreactive species of serum or blood. The electrode is preferably layered, with the layers serially deposited within a recess upon the tip of a polyamide insulated gold wire. A first glucose concentration-to-current transducing layer can be overcoated with an electrically insulating and glucose flux limiting layer (second layer) on which, optionally, an immobilized interference-eliminating horseradish peroxidase based film is deposited. An outer layer is preferably biocompatible.
336 Citations
31 Claims
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1. A glucose or lactate sensor comprising:
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one or more working electrodes; and an analyte-responsive sensing layer associated with and in electrical contact with the one or more working electrodes, the sensing layer comprising a glucose-specific or lactate-specific enzyme crosslinked with a polymer selected from the group consisting of poly(vinylimidazole), poly(vinyl pyridine), and poly(acrylamide co-I-vinylimidazole), wherein at least a portion of the sensor is adapted to be subcutaneously positioned in vivo in a subject. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19)
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20. A glucose or lactate sensor comprising:
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an electrode comprising a metal wire; and an analyte-responsive sensing layer associated with the wire, the sensing layer comprising a glucose-specific or lactate-specific enzyme crosslinked with a polymer selected from the group consisting of poly(vinylimidazole), poly(vinyl pyridine), and poly(acrylamide co-1-vinylimidazole), wherein at least a portion of the sensor is adapted to be positioned in vivo in a subject. - View Dependent Claims (21, 22)
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23. A glucose or lactate sensor comprising:
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one or more working electrodes; an analyte-responsive sensing layer associated with and in electrical contact with the one or more working electrodes, the sensing layer comprising a glucose-specific or lactate-specific enzyme crosslinked with a polymer selected from the group consisting of poly(vinylimidazole), poly(vinyl pyridine), and poly(acrylamide co-1-vinylimidazole); and at least one additional layer comprising a biocompatible layer, an interferant eliminating layer, or both; wherein at least a portion of the sensor is adapted to be positioned in vivo in a subject. - View Dependent Claims (24, 25, 26, 27, 28, 29)
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30. A glucose or lactate sensor comprising:
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one or more working electrodes; and an analyte-responsive sensing layer associated with and in electrical contact with the one or more working electrodes, the sensing layer comprising a glucose-specific or lactate-specific enzyme crosslinked with an osmium-containing polymer, wherein the osmium-containing polymer comprises poly(vinylimidazole), poly(vinyl pyridine), or poly(acrylamide co-1-vinylimidazole); and wherein at least a portion of the sensor is adapted to be positioned in vivo in a subject.
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31. A glucose or lactate sensor comprising:
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one or more working electrodes; and an analyte-responsive sensing layer associated with and in electrical contact with the one or more working electrodes, the sensing layer comprising a reaction product obtained by a process, the process comprising; (a) contacting a polymer with an osmium compound to produce an osmium-containing polymer;
wherein the polymer is poly(vinylimidazole), poly(vinyl pyridine), or poly(acrylamide co-1-vinylimidazole) and the osmium compound is [Os (bpy)2 Cl]+/2+;
[Os (4,4′
-dimethyl bipyridine)2Cl]+/2+;
[Os (4,4′
-dimethyl phenanthroline)2Cl—
]/+/2+;
[Os (4,4′
-dimethyoxy phenanthroline)2Cl]+/2+;
or [Os (4,4′
-dimethoxy bipyridine)2Cl ]+/2+; and(b) crosslinking the osmium-containing polymer with glucose oxidase or lactate oxidase to produce the reaction product; wherein at least a portion of the sensor is adapted to be positioned in vivo in a subject.
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Specification