Measurement device and method for measuring electric signal from biological sample
First Claim
1. A measurement device for measuring an electric signal from a biological sample, comprising:
- a first substrate; and
a second substrate formed on the first substrate,wherein the second substrate includes a concave portion thereby forming a flow pass between the first substrate and the second substrate,wherein the flow pass is configured to have a first end and second end, and wherein said flow pass extends in a first direction, from said first end to said second end;
a pair of introduction orifices connected to both ends of the flow pass, respectively;
a concave well positioned in the flow pass;
a reference electrode positioned in the flow pass;
at least a microelectrode formed in the well and configured to measure an electric signal from the biological sample;
a first communicating tube connected to a first portion of the well and extending in a second direction;
a second communicating tube connected to a second portion of the well and extending in a third direction;
first and second resistance baths connected to the first and second tubes, respectively; and
first and second resistors arranged in the first and second communicating tubes, respectively, or the first and second resistance baths, respectively.
2 Assignments
0 Petitions
Accused Products
Abstract
A measurement device 10 includes a measurement cell 11. The measurement cell 11 includes a substrates 17 and a substrate 16 formed on the substrate 17. The substrates 16 and 17 are bonded to each other. Thus, a flow pass 9 is formed in the measurement cell 11. As shown in FIG. 3, on the substrate 17 of the measurement cell 11, provided are a concave well 19, a protruding microelectrode 1 connected to a terminal 4a and formed on a side wall surface of the well 19, communicating tubes 15a and 15b connected to both of side surfaces of the well 19 (i.e., the flow pass 9) to face to each other, resistance baths 6a and 6b connected to the communicating tubes 15a and 15b, thin film resistors 3a and 3b arranged in the resistance baths 6a and 6b, and a reference electrode 2 connected to a terminal 4b.
16 Citations
18 Claims
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1. A measurement device for measuring an electric signal from a biological sample, comprising:
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a first substrate; and a second substrate formed on the first substrate, wherein the second substrate includes a concave portion thereby forming a flow pass between the first substrate and the second substrate, wherein the flow pass is configured to have a first end and second end, and wherein said flow pass extends in a first direction, from said first end to said second end; a pair of introduction orifices connected to both ends of the flow pass, respectively; a concave well positioned in the flow pass; a reference electrode positioned in the flow pass; at least a microelectrode formed in the well and configured to measure an electric signal from the biological sample; a first communicating tube connected to a first portion of the well and extending in a second direction; a second communicating tube connected to a second portion of the well and extending in a third direction; first and second resistance baths connected to the first and second tubes, respectively; and first and second resistors arranged in the first and second communicating tubes, respectively, or the first and second resistance baths, respectively. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15)
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16. A measurement method for measuring an electrical signal from a biological sample, the method comprising the steps of:
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a) preparing a measurement device comprising; a first substrate; and a second substrate formed on the first substrate, wherein the second substrate includes a concave portion thereby forming a flow pass between the first substrate and the second substrate, wherein the flow pass is configured to have a first end and second end, and wherein said flow pass extends in a first direction, from said first end to said second end; a pair of introduction orifices connected to both ends of the flow pass, respectively; a concave well positioned in the flow pass; a reference electrode positioned in the flow pass; at least a microelectrode formed in the well and configured to measure an electric signal from the biological sample; a first communicating tube connected to a first portion of the well and extending in a second direction; a second communicating tube connected to a second portion of the well and extending in a third direction; first and second resistance baths connected to the first and second tubes, respectively; and first and second resistors arranged in the and second communicating tubes, respectively, or the first and second resistance baths, respectively b) introducing an electrolyte solution into the flow pass by reduction of a pressure in the flow pass to fill the flow pass; c) returning the pressure in the flow pass to an atmosphere pressure; d) arranging the biological sample in the well; e) closing up the flow pass; f) applying a current to the first or second resistor to bring said at least a microelectrode in contact with the biological sample; and g) measuring an electrical signal from the biological sample. - View Dependent Claims (17, 18)
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Specification