Field-switch sequencing
First Claim
1. A method for sequencing a nucleic acid, said method comprising:
- (a) immobilizing a plurality of complexes comprising a target nucleic acid, a primer nucleic acid, and a polymerase onto a surface;
(b) applying an electric field to contact said surface with a plurality of charged nucleic acid nanoparticles comprising nucleotide phosphate (NP);
(c) removing said nucleotide phosphate (NP) from said charged nucleic acid nanoparticle, wherein said nucleotide phosphate is a substrate for said polymerase;
(d) reversing said electric field to transport unbound charged nucleic acid nanoparticles away from the surface; and
(e) detecting the incorporation of said nucleotide phosphate into a single molecule of the primer nucleic acid.
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Accused Products
Abstract
The present invention provides novel compositions, methods and apparatus for DNA sequencing that can be performed, e.g., in a two-electrode chamber. The present invention also provides a method for sequencing a nucleic acid comprising immobilizing a plurality of complexes comprising a target nucleic acid, a primer nucleic acid, and a polymerase onto a surface, contacting the surface with a plurality of charged particles comprising a nucleotide phosphate by applying an electric field, reversing the electric field to transport unbound charged particles away from the surface, and detecting the incorporation of a nucleotide phosphate into a single molecule of the primer nucleic acid.
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Citations
18 Claims
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1. A method for sequencing a nucleic acid, said method comprising:
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(a) immobilizing a plurality of complexes comprising a target nucleic acid, a primer nucleic acid, and a polymerase onto a surface; (b) applying an electric field to contact said surface with a plurality of charged nucleic acid nanoparticles comprising nucleotide phosphate (NP); (c) removing said nucleotide phosphate (NP) from said charged nucleic acid nanoparticle, wherein said nucleotide phosphate is a substrate for said polymerase; (d) reversing said electric field to transport unbound charged nucleic acid nanoparticles away from the surface; and (e) detecting the incorporation of said nucleotide phosphate into a single molecule of the primer nucleic acid. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18)
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Specification