Biological soil detector

• US 7,468,272 B2
• Filed: 10/18/2004
• Issued: 12/23/2008
• Est. Priority Date: 05/10/2004
• Status: Expired due to Fees

• Current Assignee: 3M Innovative Properties Company (3M Company)
• Original Assignee: 3M Innovative Properties Company (3M Company)
• Inventors: Landgrebe, Kevin D., Gonzalez, Bernard A., Chandrapati, Sailaja, Hovde, Bonnie J.
• Primary Examiner(s): Gitomer; Ralph
• Application Number:
  US10/967,394
• Publication Number:
  US 20050250089A1
• Time in Patent Office: 1,527 Days
• Field of Search: 435/287.4, 435/174, 435/288.5, 210/206
• US Class Current: 435/287.4
• CPC Class Codes: C12Q 1/04 Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor C12Q1/6897 takes precedence
• CPC Class Codes: C12Q 1/22 Testing for sterility conditions
• CPC Class Codes: C12Q 2326/00 Chromogens for determinations of oxidoreductase enzymes
• CPC Class Codes: C12Q 2334/00 O-linked chromogens for determinations of hydrolase enzymes, e.g. glycosidases, phosphatases, esterases
• CPC Class Codes: C12Q 2337/00 N-linked chromogens for determinations of peptidases and proteinases
• CPC Class Codes: G01N 33/521 Single-layer analytical elements

Abstract

A biological soil detector and a method for detecting bio soil are provided. The biological soil detector has a solid support member; a specific indicator immobilized on the solid support member, the specific indicator having a material sensitive to the presence of blood; and the solid support and specific indicator positioned within or on a retaining member to readily contact a liquid that has been used to contact a surface. In the method for detecting bio soil, the method includes the following steps: contacting the surface with a liquid; after the liquid has contacted the surface, contacting the liquid with the solid support member and the specific indicator of the detector described herein; and inspecting the solid support member for a detectable response to determine whether the specific indicator has interacted with biological soil.

Claims

1. A biological soil detector, comprising:

A solid support member;

A specific indicator immobilized on the solid support member, the specific indicator comprising a material sensitive to the presence of blood;

The solid support and specific indicator positioned with respect to the detector to facilitate contact with a liquid that has been used to contact a surface; and

A retaining member supporting the solid support member and the specific indicator, the retaining member positioned to facilitate contact between the liquid and the solid support member, wherein the retaining member comprises a structure which channels the liquid therethrough, the solid support and specific indicator positioned within or on the retaining member to facilitate contact between the liquid and the solid support member when the liquid is channeled through the retaining member; and

wherein the retaining member is a tubular member having a fluid inlet through which the liquid enters the retaining member and a fluid outlet through which the liquid exits the retaining member.

2. The biological soil detector of claim 1 wherein the solid support member comprises a first material selected from the group consisting of at least one polymer;

inorganic material; and

mixed organic and inorganic material.

3. The biological soil detector of claim 2 wherein the at least one polymer contains functional groups, the functional groups selected from the group consisting of carboxyl and salts thereof, aldehydes, sulfonic acid and salts thereof, phosphonic acid and salts thereof, alcohol, primary amine, secondary amine, tertiary amine, amide, imide, quaternized ammonium, sulfonium, phosphonium, pyridine, cyclic amido, oxyalkylene, imidazoles and combinations of two or more of the foregoing.

4. The biological soil detector of claim 3 wherein the at least one polymer containing functional groups comprises polymers and copolymers selected from the group consisting of carboxyl containing polymers;

polyalkoxylates;

poly(meth)acrylates;

polyvinyl alcohol;

polyethylene-vinyl alcohol copolymer;

polyurethane;

polyurea;

polyester;

polyamide;

polyimide;

polyether;

cellulose;

rayon;

polyphosphate;

polypeptide;

polyacrylonitrile;

polyacrylamide;

polycarbonate;

polyethersulfone; and

combinations of two or more of the foregoing.

5. The biological soil detector of claim 2 wherein the inorganic material is selected from the group consisting of metal oxide, metal hydrate, metal-hydroxyl and combinations of two or more of the foregoing, and wherein the metal is Si.

6. The biological soil detector of claim 2 wherein the mixed organic and inorganic material is selected from the group consisting of polymeric composites, ceramers, and combinations thereof.

7. The biological soil detector of claim 1 wherein the solid support member comprises a first material selected from the group consisting of at least one polymer, inorganic material, and mixed organic and inorganic material;

and a second material attached to the first material.

8. The biological soil detector of claim 7 wherein the first material is selected from the group consisting of polypropylene, polyethylene, polyvinylidene fluoride, tetrafluoroethylene hexafluoropropylene vinylidene fluoride, polyurethane, polyurea, polyester, polyvinyl acetate, polyamide, polyimide, poly(meth)acrylate, polyethersulfone, glass, silica, cellulosics, rayon, polycarbonate, polyvinyl alcohol, polystyrene, and combinations of the foregoing.

9. The biological soil detector of claim 8 wherein the second material is hydrophilic and is selected from the group consisting of monomer, polymer, reactive metal alkoxide and combinations of two or more of the foregoing.

10. The biological soil detector of claim 9 wherein the second material comprises one or more functional groups selected from the group consisting of carboxyl and salts thereof, aldehyde, sulfonic acid and salts thereof, phosphonic acid and salts thereof, alcohol, primary amine, secondary amine, tertiary amine, amide, imide, quaternized ammonium, sulfonium, phosphonium, pyridine, cyclic amido, oxyalkylene, ω

-saccharinamidoundecylsiloxane, glycidyl, succinimido, imidazoles and combinations of two or more of the foregoing.

11. The biological soil detector of claim 2 wherein the solid support member comprises a material selected from the group consisting of films, nonwoven material, woven material, knitted material, reticulated foam, open-celled foam, porous ceramic inorganic frit, fiber, particle-coated supports, sintered particles, sintered fiber, sponge, and polymer membrane.

12. The biological soil detector of claim 11 wherein the polymer membrane comprises a thermally induced phase separation membrane comprising, a material selected from the group consisting of high density polyethylene, polypropylene, polyvinylidenefluoride, polyethylene-vinyl copolymer and combinations of two or more of the foregoing.

13. The biological soil detector of claim 2 wherein the solid support member further comprises a hydrophilic coating.

14. The biological soil detector of claim 1 wherein the specific indicator comprises a material sensitive to one or more blood proteins.

15. The biological soil detector of claim 1 wherein the specific indicator comprises one or more compounds which react with the product of peroxide and heme.

16. The biological soil detector of claim 1 wherein the specific indicator comprises 3,3′

,5,5′

-tetramethylbenzidine.

17. The biological soil detector of claim 1, wherein the solid support and specific indicator are positioned within the retaining member between the fluid inlet and the fluid outlet.

18. The biological soil detector of claim 17 wherein the solid support and specific indicator are releasably positioned within the retaining member.

19. The biological soil detector of claim 17 further comprising a flow indicator, the flow indicator presenting a first appearance when the flow indicator is dry and a second appearance when the flow indicator has been exposed to the liquid.

20. The biological soil detector of claim 19 wherein the flow indicator is positioned near the specific indicator so that the second appearance of the flow indicator is an indication that the specific indicator has been exposed to the liquid.

21. The biological soil detector of claim 1 wherein the solid support and specific indicator are positioned on the retaining member to facilitate contact between the liquid and the solid support member when the liquid is channeled through the retaining member.

22. The biological soil detector of claim 21 wherein the solid support and specific indicator are positioned near the fluid inlet.

23. The biological soil detector of claim 17, wherein the solid support and specific indicator are releasably positioned near the fluid outlet.

24. The biological soil detector of claim 22 further comprising a flow indicator, the flow indicator presenting a first appearance when the flow indicator is dry and a second appearance when the flow indicator has been exposed to the liquid.

25. The biological soil detector of claim 24 wherein the flow indicator is positioned near the specific indicator so that the second appearance of the flow indicator is an indication that the specific indicator has been exposed to the liquid.