Simplified tissue processing
First Claim
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1. A system for tissue processing of one or more tissue specimens, wherein the system uses only two different chemical compositions, comprising:
- (a) a first module comprising (i) a first reaction chamber which is heated and has an interior geometry comprised of a whispering gallery and (ii) a single non-aqueous admixture comprised of fixing, dehydrating, and clearing functions, wherein the non-aqueous admixture is optionally contained within the first reaction chamber;
(b) a second module comprising (i) a second reaction chamber which is heated and also connected to a vacuum pump and (ii) a single wax solution, wherein the wax solution is optionally contained within the second reaction chamber; and
(c) optionally a conveyance which at least transfers the tissue specimen from the first module to the second module.
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Abstract
Improved systems and methods for tissue processing are described here. The chemical process and the construction of the apparatus are simplified by using only two different solutions in two separate reaction modules. They are compatible with processing of tissue specimens for genetic analysis, histology, in situ antibody binding and hybridization, archival preservation of morphology and nucleic acids, and combinations thereof.
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Citations
68 Claims
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1. A system for tissue processing of one or more tissue specimens, wherein the system uses only two different chemical compositions, comprising:
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(a) a first module comprising (i) a first reaction chamber which is heated and has an interior geometry comprised of a whispering gallery and (ii) a single non-aqueous admixture comprised of fixing, dehydrating, and clearing functions, wherein the non-aqueous admixture is optionally contained within the first reaction chamber; (b) a second module comprising (i) a second reaction chamber which is heated and also connected to a vacuum pump and (ii) a single wax solution, wherein the wax solution is optionally contained within the second reaction chamber; and (c) optionally a conveyance which at least transfers the tissue specimen from the first module to the second module. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34)
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35. An automated system for tissue processing of one or more tissue specimens, wherein the system has only two separate modules and only two different chemical compositions, comprising:
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(a) a first module comprising (i) a first reaction chamber having an interior geometry comprised of a whispering gallery to achieve uniform distribution of microwave energy and heating of the interior, (ii) a single non-aqueous admixture comprised of fixing, dehydrating, and clearing functions, wherein the non-aqueous admixture is optionally contained within the first reaction chamber, (iii) a source of microwave energy which is transmitted into the first reaction chamber to heat the non-aqueous admixture, and (iv) an agitator within the first reaction chamber to promote chemical exchange between the non-aqueous admixture and the tissue specimen; (b) a second module comprising (i) a second reaction chamber connected to a vacuum pump, (ii) a single wax solution, wherein the wax solution is optionally contained within the second reaction chamber, and (iii) a source of radiant energy which is transmitted into the second reaction chamber to heat the wax solution; and (c) a conveyance which at least transfers the tissue specimen from the first module to the second module. - View Dependent Claims (36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51)
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52. An automated system for tissue processing of one or more tissue specimens, wherein the system has only two separate modules and only two different chemical compositions, comprising:
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(a) a first module comprising (i) a first reaction chamber which is heated and has an interior geometry comprised of a whispering gallery and (ii) a single non-aqueous admixture comprised of at least one ketone, at least one alcohol, and mineral oil, wherein the non-aqueous admixture is optionally contained within the first reaction chamber; (b) a second module comprising (i) a second reaction chamber which is heated and also connected to a vacuum pump and (ii) a single wax solution, wherein the wax solution is optionally contained within the second reaction chamber; and (c) a conveyance which at least transfers the tissue specimen from the first module to the second module. - View Dependent Claims (53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68)
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Specification