Optical determination of glucose utilizing boronic acid adducts
First Claim
1. A device for optically determining an analyte concentration, which device comprises:
- an analyte permeable component;
a fluorophore associated with the analyte permeable component and configured to absorb light at a first wavelength and emit light at a second wavelength, wherein the fluorophore comprises a substituted pyrene sulfonate;
a quencher associated with the analyte permeable component and configured to modify the light emitted by the fluorophore by an amount related to the analyte concentration wherein the quencher comprises a boronic acid substituted viologen;
a light source; and
a detector.
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Abstract
The present invention concerns an improved optical method and optical sensing device for determining the levels of polyhydroxyl-substituted organic molecules in vitro and/or in vivo in aqueous media. The range of detection is between about 400 and 800 nm. In particular, a sensory devise is implemented in a mammal to determine sugar levels. Specifically, a dye is combined with a conjugated nitrogen-containing heterocyclic aromatic boronic acid-substituted bis-onium compound in the presence of a sugar, such as fructose or glucose. The viologens are preferred as the aromatic conjugated nitrogen-containing boronic acid substituted compounds. The method is useful to determine sugar levels in a human being.
71 Citations
32 Claims
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1. A device for optically determining an analyte concentration, which device comprises:
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an analyte permeable component; a fluorophore associated with the analyte permeable component and configured to absorb light at a first wavelength and emit light at a second wavelength, wherein the fluorophore comprises a substituted pyrene sulfonate; a quencher associated with the analyte permeable component and configured to modify the light emitted by the fluorophore by an amount related to the analyte concentration wherein the quencher comprises a boronic acid substituted viologen; a light source; and a detector. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32)
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Specification