Adipose-derived stem cells and lattices

• US 7,470,537 B2
• Filed: 12/17/2003
• Issued: 12/30/2008
• Est. Priority Date: 03/10/1999
• Status: Expired due to Term

• Current Assignee: University of Pittsburgh of The Commonwealth System of Higher Education
• Original Assignee: University of Pittsburgh of The Commonwealth System of Higher Education
• Inventors: Hedrick, Marc H., Katz, Adam J., Benhaim, Prosper, Futrell, J. William, Zhu, Min, Lorenz, Hermann Peter, Llull, Ramón
• Primary Examiner(s): Qian; Celine X
• Application Number:
  US10/740,315
• Publication Number:
  US 20050153441A1
• Time in Patent Office: 1,840 Days
• Field of Search: None
• US Class Current: 435/325
• CPC Class Codes: A61K 35/12 Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells vaccines or medicinal preparations containing antigens or antibodies A61K39/00
• CPC Class Codes: A61K 48/00 Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
• CPC Class Codes: C12N 2500/25 Insulin-transferrin; Insulin-transferrin-selenium
• CPC Class Codes: C12N 2500/38 Vitamins
• CPC Class Codes: C12N 2500/42 Organic phosphate, e.g. beta glycerophosphate
• CPC Class Codes: C12N 2501/01 Modulators of cAMP or cGMP, e.g. non-hydrolysable analogs, phosphodiesterase inhibitors, cholera toxin
• CPC Class Codes: C12N 2501/15 Transforming growth factor beta (TGF-β)
• CPC Class Codes: C12N 2501/33 Insulin
• CPC Class Codes: C12N 2501/39 Steroid hormones
• CPC Class Codes: C12N 2510/00 Genetically modified cells
• CPC Class Codes: C12N 2533/90 Substrates of biological origin, e.g. extracellular matrix, decellularised tissue
• CPC Class Codes: C12N 5/0068 General culture methods using substrates for specific animal cell type C12N5/06
• CPC Class Codes: C12N 5/0667 Adipose-derived stem cells [ADSC]; Adipose stromal stem cells

Abstract

The present invention provides adipose-derived stem cells (ADSCs), adipose-derived stem cell-enriched fractions (ADSC-EF) and adipose-derived lattices, alone and combined with the ADSCs of the invention. In one aspect, the present invention provides an ADSC substantially free of adipocytes and red blood cells and clonal populations of connective tissue stem cells. The ADSCs can be employed, alone or within biologically-compatible compositions, to generate differentiated tissues and structures, both in vivo and in vitro. Additionally, the ADSCs can be expanded and cultured to produce molecules such as hormones, and to provide conditioned culture media for supporting the growth and expansion of other cell populations. In another aspect, the present invention provides an adipose-derived lattice substantially devoid of cells, which includes extracellular matrix material from adipose tissue. The lattice can be used as a substrate to facilitate the growth and differentiation of cells, whether in vivo or in vitro, into anlagen or even mature tissues or structures.

Claims

1. An isolated population of stem cells, obtained from human adipose tissue having a CD marker profile comprising a combination of STRO-1+, CD29+, CD44+, CD71+, CD49D+, low or undetectable levels of CD 106, CD90+, and CD105+.

2. An isolated population of stem cells, obtained from human adipose tissue having a CD marker profile comprising

(a) STRO-1,

(b) CD49d,

(c) low or undetectable levels of CD106, and

(d) any one or more of CD29, CD44, CD71, CD90, SH3, and CD105, and

wherein the CD marker profile further comprises CD31−

, and CD45−

.

3. An isolated population of stem cells, obtained from human adipose tissue having a CD marker profile comprising a combination of STRO-1+, CD29+, CD44+, CD49D+, low or undetectable levels of CD106, CD71+, CD90+, CD105+, CD31−

, and CD45−

.