Method and device for the integrated synthesis and analysis of analytes on a support

• US 7,470,540 B2
• Filed: 10/17/2001
• Issued: 12/30/2008
• Est. Priority Date: 10/17/2000
• Status: Expired due to Fees

• Current Assignee: Febit AG (FEBIT)
• Original Assignee: Febit AG (FEBIT)
• Inventors: Stähler, Peer F., Stähler, Cord F., Scheffler, Matthias, Heidbrede, Anke, Güimil, Ramon
• Primary Examiner(s): Soderquist; Arlen
• Application Number:
  US10/399,450
• Publication Number:
  US 20040043509A1
• Time in Patent Office: 2,631 Days
• Field of Search: 422/58, 422/61, 422/69, 422/81, 422 8201- 8203, 422 8205- 8209, 422100-101, 422/129, 422130-131, 436/34, 436/43, 436/52, 436 86- 99, 436/104, 436164-165, 436177-178, 436524-534
• US Class Current: 436/34
• CPC Class Codes: B01J 19/0046 Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays synthesis methods per se C40B50/00
• CPC Class Codes: B01J 2219/00279 Features relating to reactor vessels
• CPC Class Codes: B01J 2219/00466 in a slurry
• CPC Class Codes: B01J 2219/00468 by manipulation of individual beads
• CPC Class Codes: B01J 2219/005 Beads
• CPC Class Codes: B01J 2219/0054 Means for coding or tagging the apparatus or the reagents
• CPC Class Codes: B01J 2219/0059 Sequential processes
• CPC Class Codes: B01J 2219/00596 Solid-phase processes
• CPC Class Codes: B01J 2219/00648 by the use of solid beads
• CPC Class Codes: B01J 2219/00657 One-dimensional arrays
• CPC Class Codes: B01J 2219/00659 Two-dimensional arrays
• CPC Class Codes: B01J 2219/00702 Processes involving means for analysing and characterising the products
• CPC Class Codes: B01J 2219/00711 Light-directed synthesis
• CPC Class Codes: B01J 2219/00722 Nucleotides
• CPC Class Codes: B01J 2219/00725 Peptides
• CPC Class Codes: B82Y 30/00 Nanotechnology for materials or surface science, e.g. nanocomposites
• CPC Class Codes: C40B 40/06 Libraries containing nucleotides or polynucleotides, or derivatives thereof
• CPC Class Codes: C40B 40/10 Libraries containing peptides or polypeptides, or derivatives thereof
• CPC Class Codes: C40B 60/14 for creating libraries
• CPC Class Codes: C40B 70/00 Tags or labels specially adapted for combinatorial chemistry or libraries, e.g. fluorescent tags or bar codes
• CPC Class Codes: G01N 33/54373 : involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
• CPC Class Codes: Y10T 436/11 : Automated chemical analysis
• CPC Class Codes: Y10T 436/117497 : with a continuously flowing sample or carrier stream
• CPC Class Codes: Y10T 436/163333 : Organic [e.g., chemical warfare agents, insecticides, etc.]
• CPC Class Codes: Y10T 436/25375 : Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.]
• CPC Class Codes: Y10T 436/255 : including use of a solid sorbent, semipermeable membrane, or liquid extraction

Abstract

A method for preparing receptor-coated particles is described which comprises the following steps:

• a) providing a support body,
• b) conducting a liquid containing particles into or onto the support body,
• c) immobilizing the particles on at least one surface area of the support body,
• d) conducting a liquid which contains receptors or receptor building blocks for synthesizing polymeric receptors over the immobilized particles,
• e) coupling the receptors or receptor building blocks location—or/and time-specifically to the immobilized particles at in each case predetermined positions of the support body,
• f) repeating, where appropriate, the steps (d) and (e), until the desired receptors have been synthesized on the immobilized particles at the in each case predetermined positions of the support body.

Claims

1. A method for integrated synthesis and analyte determination on a support, comprising the following steps:

a) providing a support body (2),

b) conducting a liquid containing particles (46) into or onto the support body (2),

c) permanently immobilizing the particles (46) on at least one inner or outer surface (45) of the support body (2),

d) conducting a liquid which contains receptors or building blocks (A, B) for synthesizing polymeric receptors over the immobilized particles (46),

e) coupling the receptors or receptor building blocks (A, B) location-specifically, time-specifically or both to the immobilized particles (46) at predetermined positions of the support body (2),

f) repeating, where appropriate, (d) and (e), until the desired receptors have been synthesized on the immobilized particles (46) at predetermined positions of the support body,

g) contacting the receptors with a sample containing the analyte to be determined, and

h) determining the analyte via binding to the receptors which are coupled to the immobilized particles (46).

2. The method as claimed in claim 1, wherein permanent immobilizing comprises a covalent chemical bond.

3. The method as claimed in claim 1, wherein the analyte is removed from the support body after the determination.

4. The method as claimed in claim 1, comprising repeating (f) a plurality of times, and wherein said receptors are synthesized for a subsequent cycle on the basis of the information from a preceding cycle.

5. The method as claimed in claim 1, wherein the support body (2) has a multiplicity of channels (40).

6. A method for integrated synthesis and analyte determination on a support, comprising the following steps:

a) providing an integrated apparatus which comprises a programmable light source matrix (4), a detector matrix (6), a support body (2) arranged between said light source matrix and said detector matrix, wherein said support body has at least some transparent regions, and a means for supplying fluid into the support body and for discharging fluid from the support body,

b) conducting a liquid containing particles (46) into or onto the support body (2),

c) permanently immobilizing the particles (46) on at least one inner or outer surface (45) of the support body (2) by a covalent chemical bond,

d) conducting a liquid which contains receptors or building blocks (A, B) for synthesizing polymeric receptors over the immobilized particles (46)

e) coupling the receptors or receptor building blocks (A, B) location-specifically, time-specifically or both to the immobilized particles (46) at predetermined positions of the support body (2),

f) repeating, where appropriate, steps (d) and (e), until the desired receptors have been synthesized on the immobilized particles (46) at the predetermined positions of the support body,

g) contacting the receptors with a sample containing the analyte to be determined, and

h) determining the analyte via binding to the receptors which are coupled to the immobilized particles (46) wherein said synthesis of the desired receptors, said determining analytes or both are monitored and controlled in at least one position on the support.