Nucleotide analogs

• US 7,476,734 B2
• Filed: 12/06/2005
• Issued: 01/13/2009
• Est. Priority Date: 12/06/2005
• Status: Expired due to Fees

• Current Assignee: Fluidigm Corporation
• Original Assignee: Helicos Biosciences Corporation
• Inventors: Liu, David R.
• Primary Examiner(s): Lewis; Patrick T
• Application Number:
  US11/295,155
• Publication Number:
  US 20070128614A1
• Time in Patent Office: 1,134 Days
• Field of Search: None
• US Class Current: 536/26.21
• CPC Class Codes: C07H 19/04 Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
• CPC Class Codes: C07H 21/04 with deoxyribosyl as saccharide radical

Abstract

The invention provides nucleotide analogs for use in sequencing nucleic acid molecules.

Claims

1. A nucleotide analog having the structure:

2. The nucleotide analog of claim 1, wherein said label is an optically-detectable label.

3. The nucleotide analog of claim 2, wherein said optically-detectable label is a fluorescent label.

4. The nucleotide analog of claim 1, wherein B is selected from the group consisting of cytosine, uracil, thymine, adenine, guanine, and analogs thereof.

5. The nucleotide analog of claim 1, wherein said linker comprises a cleavable bond.

6. The nucleotide analog of claim 5, wherein said cleavable bond is a chemically cleavable bond.

7. The nucleotide analog of claim 5, wherein said cleavable bond is a photochemically cleavable bond.

8. The nucleotide analog of claim 5, wherein said cleavable bond is cleaved upon exposure to a pH from about 11.3 to about 12.3.

9. The nucleotide analog of claim 5, wherein said cleavable bond is cleaved upon exposure to a pH of about 11.8.

10. A nucleotide analog having the structure:

11. The nucleotide analog of claim 10, wherein said label is an optically-detectable label.

12. The nucleotide analog of claim 11, wherein said optically-detectable label is a fluorescent label.