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Method for classifying a microorganism in a biological sample

  • US 7,479,375 B2
  • Filed: 01/13/2006
  • Issued: 01/20/2009
  • Est. Priority Date: 01/13/2005
  • Status: Expired due to Fees
First Claim
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1. A method of classifying a microorganism in a test sample between a Gram negative bacterium, a Gram positive bacterium, or a fungus, the method comprising the steps of:

  • (a) combining the test sample, which contains a standardized amount of the microorganism, with a hemocyte preparation to produce a mixture;

    (b) measuring either (i) an optical property of the mixture at a preselected time or (ii) a time in which a preselected change occurs in an optical property of the mixture;

    wherein the optical property is selected from the group consisting of absorbance, transmittance, optical density, and turbidity; and

    (c) using the optical property of step (b)(i) or the time value of step (b)(ii) to determine whether the microorganism in the test sample is a Gram negative bacterium, a Gram positive bacterium, or a fungus.

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