Reshaped human antibody to human interleukin-6 receptor

• US 7,479,543 B2
• Filed: 05/04/2004
• Issued: 01/20/2009
• Est. Priority Date: 04/25/1991
• Status: Expired due to Fees

• Current Assignee: Chugai Seiyaku Kabushiki Kaisha (Roche Holding AG)
• Original Assignee: Chugai Seiyaku Kabushiki Kaisha (Roche Holding AG)
• Inventors: Bendig, Mary Margaret, Tsuchiya, Masayuki, Saldanha, Jose William, Jones, Steven Tarran, Sato, Koh
• Primary Examiner(s): Yaen; Christopher H
• Application Number:
  US10/837,904
• Publication Number:
  US 20050142635A1
• Time in Patent Office: 1,722 Days
• Field of Search: None
• US Class Current: 530/387.1
• CPC Class Codes: A61K 38/00 Medicinal preparations containing peptides peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00
• CPC Class Codes: A61P 35/00 Antineoplastic agents
• CPC Class Codes: C07K 16/2866 against receptors for cytokines, lymphokines, interferons
• CPC Class Codes: C07K 2317/24 containing regions, domains or residues from different species, e.g. chimeric, humanized or veneered
• CPC Class Codes: C07K 2319/00 Fusion polypeptide

Abstract

A reshaped human antibody to the human IL-6R, comprising:

• • (A) an L chain comprising,
    • (1) a human L chain C region, and
    • (2) an L chain V region comprising human L chain framework regions (FRs), and mouse L chain complementary determination regions (CDRs) of a momoclonal antibody to the IL-6 receptor (IL-6R); and
  • (B) an H chain comprising,
    • (1) a human H chain C region, and
    • (2) an H chain V region comprising human H chain FRS, and mouse H chain CDRs of a monoclonal antibody to the IL-6R.

Since major portion of the reshaped human antibody is derived from a human antibody and the mouse CDRs which are less immunogenic, the present reshaped human antibody is less immunogenic to human, and therefor is promised for therapeutic uses.

Claims

1. A chimeric antibody to human interleukin-6 receptor (IL-6R), comprising:

(1) light chains (L chains) each comprising a human L chain constant region (C region) and an L chain variable region (V region) of a mouse monoclonal antibody to human IL-6R; and

(2) heavy chains (H chains) each comprising a human H chain C region, and H chain V region of a mouse monoclonal antibody to human IL-6R;

wherein the mouse L chain V region includes an amino acid sequence shown in SEQ ID NO;

29;

the mouse H chain V region includes an amino acid sequence shown in SEQ ID NO;

31;

the human L chain C region is a human Kc region; and

the human H chain C region is a human γ

-1C region.