Probes for a gas phase ion spectrometer

• US 7,479,631 B2
• Filed: 03/02/2007
• Issued: 01/20/2009
• Est. Priority Date: 04/27/1999
• Status: Expired due to Term

• Current Assignee: Bio-Rad Laboratories Incorporated
• Original Assignee: Bio-Rad Laboratories Incorporated
• Inventors: Um, Pil-Je, Rich, William E., Beecher, Jody, Voivodov, Kamen, Yip, Tai-Tung
• Primary Examiner(s): Wells; Nikita
• Application Number:
  US11/681,377
• Publication Number:
  US 20070158547A1
• Time in Patent Office: 690 Days
• Field of Search: 250/288, 250/281, 250/282, 436/173, 428/333, 428/411.1
• US Class Current: 250/288
• CPC Class Codes: H01J 49/0418 for laser desorption, e.g. matrix-assisted laser desorption/ionisation [MALDI] plates or surface enhanced laser desorption/ionisation [SELDI] plates
• CPC Class Codes: H01J 49/12 using an arc discharge, e.g. of the duoplasmatron type
• CPC Class Codes: Y10T 428/261 In terms of molecular thickness or light wave length
• CPC Class Codes: Y10T 428/31504 Composite [nonstructural laminate]
• CPC Class Codes: Y10T 436/24 Nuclear magnetic resonance, electron spin resonance or other spin effects or mass spectrometry

Abstract

The invention provides a probe and a method of making the probe that is removably insertable into a gas phase ion spectrometer, the probe comprising a substrate having a surface and a hydrogel material on the surface, the hydrogel material comprising binding functionalities for binding with an analyte detectable by the gas phase ion spectrometer. The invention also provides a probe and a method of making the probe that is removably insertable into a gas phase ion spectrometer, the probe comprising a substrate having a surface and a plurality of particles that are uniform in diameter on the surface, the particles comprising binding functionalities for binding with an analyte detectable by the gas phase ion spectrometer. Further, the invention provides a system comprising the probe of the present invention and a gas phase ion spectrometer comprising an energy source that directs light to the probe surface to desorb an analyte and a detector in communication with the probe surface that detects the desorbed analyte. The invention also provides a method for desorbing an analyte from a probe surface, the method comprising exposing the binding functionalities to a sample containing an analyte under conditions to allow binding between the analyte and the binding functionalities, and desorbing the analyte from the probe by gas phase ion spectrometry.

Claims

1. A probe that is removably insertable into a gas phase ion spectrometer, the probe comprising a substrate having a surface and a hydrogel material on the surface, wherein the hydrogel material is crosslinked and comprises binding functionalities for binding with an analyte detectable by the gas phase ion spectrometer.

2. A probe that is removably insertable into a gas phase ion spectrometer, the probe comprising a substrate having a surface and a plurality of particles that are substantially uniform in diameter on the surface, the particles comprising binding functionalities for binding with an analyte detectable by the gas phase ion spectrometer.

3. The probe of claim 2 wherein the plurality of particles have an average diameter of less than about 1000 μ

m.

4. The probe of claim 2 wherein the particles have a coefficient of diameter variation of less than about 5%.

5. The probe of claim 2 wherein the surface of the substrate is conditioned to adhere to the particles.

6. The probe of claim 2 wherein the binding functionalities of the particles are selected from the group consisting of a carboxyl group, a sulfonate group, a phosphate group, an ammonium group, a hydrophilic group, a hydrophobic group, a reactive group, a metal chelating group, a thioether group, a biotin group, a boronate group, a dye group, a cholesterol group, and derivatives thereof.

7. A system for detecting an analyte comprising:

a gas phase ion spectrometer comprising an inlet system, and

a removably insertable probe inserted into the inlet system of the gas phase ion spectrometer, the probe comprising a substrate having a surface and a hydrogel material on the surface, wherein the hydrogel material is crosslinked and comprises binding functionalities for binding with the analyte.

8. The system of claim 7 wherein the gas phase ion spectrometer is a mass spectrometer.

9. The system of claim 8, wherein the mass spectrometer is a laser desorption mass spectrometer.

10. The system of claim 9 wherein the substrate is in the form of a strip or a plate.

11. The system of claim 9 wherein the hydrogel material is in situ polymerized on the surface of the substrate by depositing a solution comprising monomers onto the substrate surface, wherein the monomers are pre-functionalized to provide binding functionalities.

12. A system for detecting an analyte comprising:

a gas phase ion spectrometer comprising an inlet system; and

a removably insertable probe that is inserted into the inlet system of the gas phase ion spectrometer, the probe comprising a substrate having a surface and a plurality of particles that are substantially uniform in diameter on the surface, the particles comprising binding functionalities for binding with the analyte.

13. The system of claim 12 wherein the gas phase ion spectrometer is a mass spectrometer.

14. The system of claim 13 wherein the mass spectrometer is a laser desorption mass spectrometer.

15. The system of claim 14 wherein the plurality of particles have an average diameter of less than about 1000 μ

m.

16. The system of claim 14 wherein the particles have a coefficient of diameter variation of less than about 5%.

17. A method of making a probe that is removably insertable into a gas phase ion spectrometer, the method comprising:

providing a substrate having a surface;

conditioning the surface of the substrate; and

placing a hydrogel material on the surface of the substrate, wherein the hydrogel material is crosslinked and comprises binding functionalities for binding with an analyte detectable by the gas phase ion spectrometer.

18. The method of claim 17 wherein the surface of the substrate is conditioned by roughening.

19. The method of claim 17 wherein the surface of the substrate is conditioned by laser etching, chemical etching, or sputter etching.

20. The method of claim 17 wherein the surface of the substrate is conditioned by incorporating a metal coating, an oxide coating, a sol gel, a glass coating, or a coupling agent.

21. The method of claim 17 wherein the hydrogel material is produced by polymerizing monomers in situ on the surface of the substrate.

22. The method of claim 21, wherein the monomers are pre-functionalized to provide binding functionalities.

23. The method of claim 17 wherein the binding functionalities are selected from the group consisting of a carboxyl group, a sulfonate group, a phosphate group, an ammonium group, a hydrophilic group, a hydrophobic group, a reactive group, a metal chelating group, a thioether group, a biotin group, a boronate group, a dye group, a cholesterol group, and derivatives thereof.

24. The method of claim 17 wherein the hydrogel material is crosslinked by irradiation.

25. The method of claim 17 wherein the hydrogel material is produced by crosslinking monomers by irradiation in situ on the surface of the substrate.

26. A method of making a probe that is removably insertable into a gas phase ion spectrometer, the method comprising:

providing a substrate with a surface;

conditioning the surface of the substrate; and

placing a plurality of particles that are substantially uniform in diameter on the surface of the substrate, the particles comprising binding functionalities for binding with an analyte detectable by the gas phase ion spectrometer.

27. The method of claim 26 wherein the surface of the substrate is conditioned by roughening.

28. The method of claim 26 wherein the surface of the substrate is conditioned by laser etching, chemical etching, or sputter etching.

29. The method of claim 26 wherein the surface of the substrate is conditioned by a crosslinking reagent so that particles can be covalently bonded to the surface of the substrate.

30. A method for detecting an analyte comprising:

(a) providing a probe that is removably insertable into a gas phase ion spectrometer, the probe comprising a substrate having a surface and a hydrogel material on the surface, wherein the hydrogel material is crosslinked and comprises binding functionalities for binding with the analyte;

(b) exposing the binding functionalities of the hydrogel material to a sample containing an analyte under conditions to allow binding between the analyte and the binding functionalities of the hydrogel material;

(c) striking the probe surface with energy from an ionization source;

(d) desorbing the bound analyte from the probe by the gas phase ion spectrometer; and

(e) detecting the desorbed analyte.