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Methods for detection of a target nucleic acid by capture

  • US 7,482,121 B2
  • Filed: 04/14/2005
  • Issued: 01/27/2009
  • Est. Priority Date: 10/29/1999
  • Status: Expired due to Fees
First Claim
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1. A method of generating a signal indicative of the presence of a target nucleic acid sequence in a sample, comprisingannealing two non-overlapping oligonucleotides to the same strand of said target nucleic acid sequence so as to form a cleavage structure, wherein said cleavage structure comprises duplex and single-stranded nucleic acid, said single stranded nucleic acid comprising a 5′

  • flap that does not specifically anneal to said target nucleic acid sequence, andcleaving said cleavage structure with a thermostable FEN- 1 nuclease so as to generate a signal, wherein generation of said signal is indicative of the presence of a target nucleic acid sequence in said sample.

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