Anti-feline albumin antibodies

• US 7,482,128 B2
• Filed: 03/25/2004
• Issued: 01/27/2009
• Est. Priority Date: 03/27/2003
• Status: Expired due to Fees

• Current Assignee: Heska Corporation (Mars Incorporated)
• Original Assignee: Heska Corporation (Mars Incorporated)
• Inventors: Andrews, Janet S., Jensen, Wayne A., Hunter, Shirley Wu, Sverlow, Karen
• Primary Examiner(s): Shibuya; Mark L
• Assistant Examiner(s): CHEU, CHANGHWA J
• Application Number:
  US10/550,563
• Publication Number:
  US 20060281131A1
• Time in Patent Office: 1,769 Days
• Field of Search: 435/7.1, 435/7.2, 435/7.91, 435/7.92, 436/516, 436/518
• US Class Current: 435/7.1
• CPC Class Codes: C07K 16/18 against material from animals or humans
• CPC Class Codes: G01N 2333/765 Serum albumin, e.g. HSA
• CPC Class Codes: G01N 2800/347 Renal failures; Glomerular diseases; Tubulointerstitial diseases, e.g. nephritic syndrome, glomerulonephritis; Renovascular diseases, e.g. renal artery occlusion, nephropathy
• CPC Class Codes: G01N 2800/52 Predicting or monitoring the response to treatment, e.g. for selection of therapy based on assay results in personalised medicine; Prognosis
• CPC Class Codes: G01N 33/6827 Total protein determination, e.g. albumin in urine
• CPC Class Codes: G01N 33/6893 related to diseases not provided for elsewhere

Abstract

The present invention provides isolated monoclonal antibodies that selectively bind albumin from animals. Also provided are methods using such antibodies for the detection of early renal disease in animals. The method includes the steps of (a) obtaining a sample from an animal to be tested; (b) contacting the sample with an antibody having a greater avidity for feline albumin than for other proteins or components in the sample; (c) detecting the complex formed by the antibody and albumin; and (d) determining the amount of albumin in the sample from the amount of antibody-albumin complex detected. An amount of albumin in the range of from 10 μg/ml to about 300 μg/ml indicates the presence of early renal disease.

Claims

1. An isolated monoclonal antibody that selectively binds albumin from a fetid or a canid, wherein said antibody binds the same epitope recognized by the monoclonal antibody produced by the hybridoma cell line having ATCC Accession No. PTA-9498 (MAb H425).

2. A kit comprising:

(a) an isolated monoclonal antibody that selectively binds albumin from a feud or canid, wherein said antibody binds the same epitope recognized by the monoclonal antibody produced by the hybridoma cell line having ATCC Accession No. PTA-9498 (MAb H425); and

(b) a means for detecting the amount of albumin in a sample collected from a felid or canid, wherein said means detects albumin in a range from about 10 μ

g/ml to about 50 μ

g/ml.