Nucleic acid detection cassette and nucleic acid detection device

• US 7,482,153 B2
• Filed: 03/21/2006
• Issued: 01/27/2009
• Est. Priority Date: 03/18/2004
• Status: Expired due to Fees

• Current Assignee: Kabushiki Kaisha Toshiba (Toshiba Corporation)
• Original Assignee: Kabushiki Kaisha Toshiba (Toshiba Corporation)
• Inventors: Ooki, Kenji, Okada, Jun, Hongo, Sadato
• Primary Examiner(s): Griffin; Walter D
• Assistant Examiner(s): Edwards; Lydia
• Application Number:
  US11/384,506
• Publication Number:
  US 20060216812A1
• Time in Patent Office: 1,043 Days
• Field of Search: 435/287.2, 435/287.5
• US Class Current: 435/287.2
• CPC Class Codes: B01L 2200/027 for microfluidic devices
• CPC Class Codes: B01L 2200/04 Exchange or ejection of cartridges, containers or reservoirs
• CPC Class Codes: B01L 2200/10 Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
• CPC Class Codes: B01L 2200/16 Reagents, handling or storing thereof
• CPC Class Codes: B01L 2300/044 pierceable, e.g. films, membranes
• CPC Class Codes: B01L 2300/0636 Integrated biosensor, microarrays
• CPC Class Codes: B01L 2300/0645 Electrodes
• CPC Class Codes: B01L 2300/0809 rectangular shaped
• CPC Class Codes: B01L 2300/0861 Configuration of multiple channels and/or chambers in a single devices
• CPC Class Codes: B01L 2300/0874 Three dimensional network
• CPC Class Codes: B01L 2400/0478 pistons
• CPC Class Codes: B01L 2400/0481 squeezing of channels or chambers
• CPC Class Codes: B01L 2400/0487 fluid pressure, pneumatics
• CPC Class Codes: B01L 3/502715 characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
• CPC Class Codes: B01L 3/502738 characterised by integrated valves microvalves F16K99/0001

Abstract

A nucleic acid detection cassette includes a cassette body, a nucleic acid detection region disposed in the cassette body, a first channel disposed in the cassette body, a second channel disposed in the cassette body. The nucleic acid detection region, in which a nucleic acid probe is immobilized, has a reagent inflow port, to which the first channel is connected, and a reagent outflow port, to which the second channel is connected. The nucleic acid detection cassette further includes a reagent injection portion which injects a reagent into the first channel, and a nucleic acid pretreatment region which is disposed in the first channel and which performs pretreatment for the detection of a nucleic acid. The first channel, the second channel, the nucleic acid detection region, the nucleic acid pretreatment region, and the reagent injection portion are sealed.

Claims

1. A nucleic acid detection cassette comprising:

a cassette body;

a nucleic acid detection region which is disposed in the cassette body and which has a reagent inflow port and a reagent outflow port and in which a nucleic acid probe is immobilized;

a first channel which is disposed in the cassette body and which is connected to the reagent inflow port of the nucleic acid detection region;

a second channel which is disposed in the cassette body and which is connected to the reagent outflow port of the nucleic acid detection region;

a reagent injection portion which injects a reagent into the first channel; and

a nucleic acid pretreatment region which is disposed in the first channel and which performs pretreatment for the detection of a nucleic acid,

the first channel, the second channel, the nucleic acid detection region, the nucleic acid pretreatment region, and the reagent injection portion being sealed, the first and second channels being connected to each other to form a circulation channel of the first channel, the second channel, the nucleic acid detection region, the nucleic acid pretreatment region, and the reagent injection portion.

2. The nucleic acid detection cassette according to claim 1, wherein the nucleic acid pretreatment region includes a region which performs a nucleic acid amplifying.

3. The nucleic acid detection cassette according to claim 2, wherein the nucleic acid pretreatment region further includes a region which performs a nucleic acid extracting.

4. The nucleic acid detection cassette according to claim 1, wherein the first channel and the second channel constitute part of a pump which moves a fluid including the reagent.

5. The nucleic acid detection cassette according to claim 1, further comprising a pump which moves a fluid including the reagent, wherein the first channel and the second channel are connected through the pump.

6. The nucleic acid detection cassette according to claim 1, further comprising a valve disposed in a channel including the first channel and the second channel.

7. The nucleic acid detection cassette according to claim 5, wherein the first channel includes two channels which branch and recombine in the nucleic acid pretreatment region, the nucleic acid pretreatment region includes a sample chamber which contains a solution, the sample chamber includes a reagent containing portion and a buffer pipe, the reagent containing portion has a lower end portion which is connected to one of the branched channels, and the buffer pipe has an end which is connected to an upper end portion of the reagent containing portion and another end which is connected to the other branched channel.

8. The nucleic acid detection cassette according to claim 5, further comprising a third channel disposed in the cassette body, branched from the first channel between the nucleic acid pretreatment region and the nucleic acid detection region, and combined with the second channel between the nucleic acid detection region and the pump;

a valve disposed in the third channel; and

a valve disposed in the first channel between a branching portion to the third channel and the nucleic acid detection region.

9. The nucleic acid detection cassette according to claim 5, further comprising a waste liquid chamber disposed in and connected to the second channel.

10. The nucleic acid detection cassette according to claim 5, wherein the nucleic acid pretreatment region includes a nucleic acid extraction region which performs a nucleic acid extracting and a nucleic acid amplification region which performs a nucleic acid amplifying, and the nucleic acid extraction region and the nucleic acid amplification region are disposed in order from the pump to the nucleic acid detection region in the first channel.

11. The nucleic acid detection cassette according to claim 10, wherein the nucleic acid pretreatment region further includes a nucleic acid modification region which performs a nucleic acid modifying, and the nucleic acid modification region is disposed in the first channel between the nucleic acid amplification region and the nucleic acid detection region.

12. The nucleic acid detection cassette according to claim 5, wherein the nucleic acid detection cassette further includes a valve disposed in a channel including the first channel and the second channel, further comprising a valve controller which controls the valve of the nucleic acid detection cassette.

13. The nucleic acid detection cassette according to claim 1, further comprising a region to store a detection reagent, which is introduced into the nucleic acid detection region.