×

Determination of vitamin D metabolite and displacement from plasma or serum binding proteins

  • US 7,482,162 B2
  • Filed: 12/06/2001
  • Issued: 01/27/2009
  • Est. Priority Date: 12/06/2000
  • Status: Expired due to Fees
First Claim
Patent Images

1. A method for measuring vitamin D metabolite present in a plasma or serum sample, the method comprising:

  • (a) adding to a serum or plasma sample a non-competitive displacement agent comprising 8-anilino-1-napthalenesulfonic acid ammonium salt, 3-(acetonylbenzyl)-4-hydroxycoumarin and a water miscible solvent, which separates any vitamin D metabolite in the sample from protein to which the vitamin D metabolite is bound, such that any separated vitamin D metabolite can be captured;

    (b) contacting the sample with a support having immobilised thereon a binding factor, the binding factor capturing any vitamin D metabolite separated in step (a); and

    (c) measuring the amount of vitamin D metabolite captured in step (b);

    wherein capture of separated vitamin D metabolite occurs in step (b) without prior extraction from the sample of the protein separated from the vitamin D metabolite in step (a).

View all claims
  • 1 Assignment
Timeline View
Assignment View
    ×
    ×