Microfluidic device and method of locally concentrating electrically charged substances in a microfluidic device

• US 7,485,214 B2
• Filed: 12/17/2004
• Issued: 02/03/2009
• Est. Priority Date: 12/23/2003
• Status: Expired due to Fees

• Current Assignee: STMicroelectronics SRL (STMicroelectronics NV)
• Original Assignee: STMicroelectronics SRL (STMicroelectronics NV)
• Inventors: Palmieri, Michele
• Primary Examiner(s): Nguyen; Nam X
• Assistant Examiner(s): Dinh; Bach T
• Application Number:
  US11/015,633
• Publication Number:
  US 20050155860A1
• Time in Patent Office: 1,509 Days
• Field of Search: 137/828, 438/49, 438/50, 438/51, 438/52, 438/22, 435/283.1, 435/287.2, 257/536, 257/382, 257/14, 204/454, 204/451, 204/601, 204/600
• US Class Current: 204/660
• CPC Class Codes: B01L 2200/0647 Handling flowable solids, e.g. microscopic beads, cells, particles
• CPC Class Codes: B01L 2300/0887 Laminated structure
• CPC Class Codes: B01L 2400/0415 electrical forces, e.g. electrokinetic
• CPC Class Codes: B01L 3/502707 characterised by the manufacture of the container or its components by shaping or joining plastic parts B29C59/00, B29C65/00; by laminating B32B37/00; manufacture of microstructural devices in general B81C
• CPC Class Codes: B01L 3/502753 characterised by bulk separation arrangements on lab-on-a-chip devices, e.g. for filtration or centrifugation separation in general B01D; microapparatus for analysis using electrophoresis G01N27/44791; sample preparation G01N1/28
• CPC Class Codes: B03C 5/026 using open-gradient differential dielectric separation, i.e. using electrodes of special shapes for non-uniform field creation, e.g. Fluid Integrated Circuit [FIC]
• CPC Class Codes: G01N 2001/4038 electric methods, e.g. electromigration, electrophoresis, ionisation

Abstract

A microfluidic device includes a microfluidic circuit, having an axis, and an electric field generator, arranged to establish an electric field (E) within at least a section of the microfluidic circuit, the electric field (E) being oriented transversally to the axis. The electric field is used to locally concentrate charged molecules, thus increasing the reaction rate.

Claims

1. A microfluidic device, comprising:

a) a microfluidic circuit having longitudinal axis; and

b) an electric field generating means arranged to establish an electric field transverse to said longitudinal axis within at least a section of said microfluidic circuit;

wherein said electric field generating means comprises;

i) a first semiconductor region having a first type of conductivity and a second semiconductor region having an opposite type of conductivity; and

ii) a voltage source for supplying a bias voltage between said first and said second semiconductor regions in order to concentrate an electrically charged substance in a portion of the microfluidic circuit; and

iii) wherein at least said section of said microfluidic circuit is between said first semiconductor region and said second semiconductor region, and wherein said first and second semiconductor regions being arranged to form at least one P-N junction.

2. The microfluidic device of claim 1, wherein said microfluidic circuit extends within said first semiconductor region and is upwardly delimited by said second semiconductor region.

3. The microfluidic device of claim 1, wherein said first semiconductor region comprises a substrate.

4. The microfluidic device of claim 3, wherein said second semiconductor region comprises at least a portion of a structural layer.

5. The microfluidic device of claim 1, wherein said first and said second semiconductor regions extend along the whole length of said section of said microfluidic circuit.

6. The microfluidic device of claim 1, wherein said voltage source is a negative voltage source.

7. The microfluidic device of claim 1, wherein said microfluidic circuit is formed as a microfluidic channel.

8. The microfluidic device of claim 7, wherein said channel is a buried channel.

9. A method for increasing the concentration of electrically charged substances in a microfluidic device, comprising the steps of:

a) applying an electrically charged substance to a microfluidic circuit of a microfluidic device, said microfluidic circuit having longitudinal axis and being between a first semiconductor region having a first type of conductivity and a second semiconductor region having an opposite type of conductivity;

b) providing a bias voltage between said first and second semiconductor regions;

c) thereby establishing an electric field within at least a section of said microfluidic circuit, said electric field being oriented transversally to said longitudinal axis, and thereby concentrating said electrically charged substance;

wherein said first and second semiconductor regions are arranged to form at least one P-N junction and providing a bias voltage comprises reverse biasing said P-N junction.

10. The method of claim 9, wherein the electrically charged substance is nucleic acid.

11. The method of claim 9, wherein the electrically charged substance is DNA.