Post-termination labeling of nucleic acid fragments and uses thereof

• US 7,485,417 B2
• Filed: 11/22/2002
• Issued: 02/03/2009
• Est. Priority Date: 06/24/1998
• Status: Expired due to Fees

• Current Assignee: Enzo Diagnostics, Inc.
• Original Assignee: Enzo Life Sciences Incorporated (Enzo Biochem Incorporated)
• Inventors: Stavrianopoulos, Jannis G., Donegan, James J., Rabbani, Elazar, Coleman, Jack, Walner, Marleen
• Primary Examiner(s): Horlick; Kenneth R.
• Assistant Examiner(s): Thomas; David C
• Application Number:
  US10/304,035
• Publication Number:
  US 20030104460A1
• Time in Patent Office: 2,265 Days
• Field of Search: 435/6, 435/91.2, 536/23.1
• US Class Current: 435/6.12
• CPC Class Codes: C12P 19/34 Polynucleotides, e.g. nucleic acids, oligoribonucleotides
• CPC Class Codes: C12Q 1/6844 Nucleic acid amplification reactions
• CPC Class Codes: C12Q 1/6853 using modified primers or templates
• CPC Class Codes: C12Q 1/6869 Methods for sequencing
• CPC Class Codes: C12Q 2525/161 incorporating target specific and non-target specific sites
• CPC Class Codes: C12Q 2525/301 Hairpin oligonucleotides
• CPC Class Codes: C12Q 2531/119 Strand displacement amplification [SDA]

Abstract

This invention provides novel processes for amplifying nucleic acid sequences of interest, including linear and non-linear amplification. In linear amplification, a single initial primer or nucleic acid construct is utilized to carry out the amplification process. In non-linear amplification, a first initial primer or nucleic acid construct is employed with a subsequent initial primer or nucleic acid construct. In other non-linear amplification processes provided by this invention, a first initial primer or nucleic acid construct is deployed with a second initial primer or nucleic acid construct to amplify the specific nucleic acid sequence of interest and its complement that are provided. A singular primer or a singular nucleic acid construct capable of non-linear amplification can also be used to carry out non-linear amplification in accordance with this invention. Post-termination labeling process for nucleic acid sequencing is also disclosed in this invention that is based upon the detection of tagged molecules that are covalently bound to chemically reactive groups provided for chain terminators. A process for producing nucleic acid sequences having decreased thermodynamic stability to complementary sequences is also provided and achieved by this invention. Unique nucleic acid polymers are also disclosed and provided in addition to other novel compositions, kits and the like.

Claims

1. A process for non-linearly amplifying a specific target nucleic acid sequence comprising the steps of:

(1) providing;

said specific target nucleic acid sequence;

a primer or a singular nucleic acid construct for non-linear amplification, comprising three segments;

(a) a first segment being sufficiently complementary to a first portion of said specific target nucleic acid sequence to (i) bind or hybridize thereto and (ii) provide for template-dependent extension of said first portion such that a first primer extension is produced;

(b) a second segment sufficiently identical to a second portion of said specific target nucleic acid sequence to provide sequences that bind to sequences produced by extension of said first segment of (a); and

(c) a third segment sufficiently identical to said first segment of (a) that use of said third segment as a template directs synthesis of a complementary sequence that can then be further extended by either (i) using itself as template in an intramolecular extension or (ii) using an unextended primer as a template in an intermolecular extension, and

(d) substrates, buffer and a template-dependent polymerizing enzyme; and

(2) incubating said specific target nucleic acid sequence and said primer or nucleic acid construct in the presence of said substrates, buffer and template-dependent polymerizing enzyme to obtain said non-linearly amplified target sequence, provided that at least one primer extension is carried out under limited cycling conditions, limited substrate conditions, limited extension duration or a combination of any of the foregoing.

2. The process of claim 1, wherein the first, second and third segments are contiguous segments.

3. The process of claim 1, wherein said primer or singular nucleic acid construct comprises a nucleic acid selected from the group consisting of a linear nucleic acid, branched nucleic acid, an inverted nucleic acid and a peptide-nucleic acid, or a combination of any of the foregoing.

4. The process of claim 1, wherein said specific target nucleic acid sequence is in single-stranded form.

5. The process of claim 1, wherein said specific target nucleic acid sequence is found or contained in a fragment.

6. The process of claim 5, wherein said fragment is produced by a means selected from the group consisting of physical means, chemical means, physio-chemical means and enzymatic means, or combinations thereof.

7. The process of claim 6, wherein said physical means are selected from the group consisting of sonication and heat, or both.

8. The process of claim 6, wherein said chemical means comprise acid treatment.

9. The process of claim 6, wherein said enzymatic means is carried out by or with nucleases and restriction enzymes.

10. The process of claim 9, wherein said nucleases comprise endonucleases.

11. The process of claim 1, wherein said first, second or third segment or their primer extensions, or any combination thereof, comprises at least one modified nucleotide or nucleotide analog.

12. The process of claim 6, wherein said modified nucleotide or nucleotide analog comprises a negatively charged chemical group.

13. The process of claim 6, wherein said negatively charged chemical group comprises carboxylic acid.

14. The process of claim 1, further comprising labeling said amplified nucleic acid sequence with a tagged molecule and a terminator thereby obtaining an amplified nucleic acid sequence comprising a tagged molecule and a terminator.

15. The process of claim 14, wherein said tagged molecule is selected from the group consisting of fluorescent dyes, chemiluminescent dyes, infra-red dyes, chemiluminescent entities and electrochemiluminescent entities, or combinations thereof.

16. The process of claim 14, wherein said terminator is a dideoxynucleotide.

17. The process of claim 14, wherein said terminator comprises a modified sugar ring.

18. The process of claim 14, wherein said terminator comprises an amino group at the 3′

position.

19. The process of claim 1, further comprising a separating step which comprises isolating said amplified nucleic acid sequence.

20. The process of claim 19, wherein said separating step is carried out electrophoretically and comprises a gel.

21. The process of claim 20, wherein said gel comprises a polyacrylamide gel.

22. The process of claim 19, wherein said separating step is carried out by capillary gel electrophoresis.

23. The process of claim 1, further comprising a detecting step, comprising detecting said amplified nucleic acid sequence, wherein said detecting step is carried out by a means selected from photometric measurement, spectrophotometric measurement, colorimetric measurement, fluorometric measurement, delayed fluorescent measurement and chemiluminescent measurement, or combinations thereof.