Diffraction-based diagnostic devices
First Claim
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1. A method for producing biosensors, comprising:
- forming a blocking layer of thiol-containing molecules on a metalized substrate member, the thiol-containing molecules forming thiol-metal bonds with the metal surface of the substrate member;
exposing the substrate member to an oxidizing enhancing stimulus through a mask having a pattern of exposed areas and protected areas, said exposure being of sufficient time to oxidize the thiol-metal bonds in the exposed areas of the blocking layer under the mask;
exposing the substrate member to a first source of thiolated biomolecules without an intervening washing step, for a time sufficient to remove the oxidized thiol-metal bonds in the exposed areas and form a layer of the first thiolated biomolecules directly on the metalized substrate member at the oxidized areas of the blocking layer by displacing the oxidized thiol-containing molecules in the exposed areas with the first thiolated biomolecule, the first thiolated biomolecules being specific for an analyte of interest having a particular size, the first thiolated biomolecules defining active areas of receptive material in a pattern corresponding to the oxidized areas defined by the exposing to oxidize step, wherein when the biosensor is exposed to a medium containing the analyte of interest, the analyte binds to the receptive material in the active areas such that the particular size of the analyte of interest, with or without a diffraction enhancing element, facilitates subsequent diffraction of light in a diffraction pattern corresponding to the active areas.
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Abstract
A biosensor includes a substrate with areas of active receptive material disposed thereon. The receptive material is specific for an analyte of interest. A pattern of the active areas is defined on the substrate by an oxidizing photo-masking process.
188 Citations
19 Claims
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1. A method for producing biosensors, comprising:
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forming a blocking layer of thiol-containing molecules on a metalized substrate member, the thiol-containing molecules forming thiol-metal bonds with the metal surface of the substrate member; exposing the substrate member to an oxidizing enhancing stimulus through a mask having a pattern of exposed areas and protected areas, said exposure being of sufficient time to oxidize the thiol-metal bonds in the exposed areas of the blocking layer under the mask; exposing the substrate member to a first source of thiolated biomolecules without an intervening washing step, for a time sufficient to remove the oxidized thiol-metal bonds in the exposed areas and form a layer of the first thiolated biomolecules directly on the metalized substrate member at the oxidized areas of the blocking layer by displacing the oxidized thiol-containing molecules in the exposed areas with the first thiolated biomolecule, the first thiolated biomolecules being specific for an analyte of interest having a particular size, the first thiolated biomolecules defining active areas of receptive material in a pattern corresponding to the oxidized areas defined by the exposing to oxidize step, wherein when the biosensor is exposed to a medium containing the analyte of interest, the analyte binds to the receptive material in the active areas such that the particular size of the analyte of interest, with or without a diffraction enhancing element, facilitates subsequent diffraction of light in a diffraction pattern corresponding to the active areas. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19)
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Specification