Method for attachment of silylated molecules to glass surfaces

• US 7,485,469 B2
• Filed: 01/19/2007
• Issued: 02/03/2009
• Est. Priority Date: 05/28/2002
• Status: Expired due to Term

• Current Assignee: Nanosphere Incorporated (DiaSorin S.p.A.)
• Original Assignee: Nanosphere Incorporated (DiaSorin S.p.A.)
• Inventors: Garimella, Viswanadham, Bernal, Yasmith
• Primary Examiner(s): Riley; Jezia
• Application Number:
  US11/656,219
• Publication Number:
  US 20070292843A1
• Time in Patent Office: 746 Days
• Field of Search: 436/518, 436/524, 436/527, 436/106, 436/109, 436/127, 435/4, 435/6, 435/174
• US Class Current: 436/518
• CPC Class Codes: C03C 17/30 with silicon-containing compounds
• CPC Class Codes: C12Q 1/6834 Enzymatic or biochemical coupling of nucleic acids to a solid phase
• CPC Class Codes: G01N 33/552 Glass or silica
• CPC Class Codes: Y10T 436/17 Nitrogen containing
• CPC Class Codes: Y10T 436/172307 Cyanide or isocyanide
• CPC Class Codes: Y10T 436/20 Oxygen containing

Abstract

A method for the efficient immobilization of silylated molecules such as silylated oligonucleotides or proteins onto unmodified surfaces such as a glass surface is provided. Also provided are compounds, devices, and kits for modifying surfaces such as glass surfaces.

Claims

1. A compound having a formula v:

(R₁)(R₂)(R₃)Si—

X-Z-CYNH—

Si(NHCYL-M)₃ 

 

v

wherein R₁, R₂ and R₃ independently represents C₁-C₆ alkoxy, C₁-C₆ alkyl, phenyl, or aryl substituted with one or more groups selected from the group consisting of C₁-C₆ alkyl and C₁-C₆ alkoxy;

L represents a linking group;

X represents linear or branched C₁-C₂₀ alkyl or aryl substituted with one or more groups selected from the group consisting of C₁-C₆ alkyl and C₁-C₆ alkoxy, optionally substituted with one or more heteroatoms comprising oxygen, nitrogen, or sulfur;

Y represents oxygen or sulfur; and

Z represents oxygen or NH; and

M represents a molecule, with the proviso that at least one of R₁, R₂, or R₃ represents C₁-C₆ alkoxy.

2. The compound of claim 1 wherein L represents a nucleophilic group from the molecule.

3. The compound of claim 2 wherein the nucleophilic group comprises —

NH, —

S—

, —

O—

, or —

OOC—

.

4. The compound of claim 1, wherein the molecule comprises a protein, a peptide, a nucleic acid, a peptide nucleic acid, an amino acid, a linked nucleic acid, a nucleoside triphosphate, a carbohydrate, a lipid, a lipid bound protein, an aptamer, a virus, a cell fragment, or a whole cell.

5. The compound of claim 1, wherein the lipid bound protein comprises a G-protein coupled receptor.

6. The compound of claim 1, wherein the molecule comprises an antibody, an antigen, a receptor, or a ligand.