Simple method for quantitative measuring the adhesion of platelets ex vivo
First Claim
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1. A diagnostic method for measuring the adhesion of platelets ex vivo comprisinga) selecting an amount and type of monodisperse polymer particles derived from at least one artificial polymer;
- b) providing a sample of whole blood;
c) adding said monodisperse polymer particles to the sample of whole blood to form a blood/polymer particles composition;
d) measuring the number of platelets in the whole blood/polymer particles composition;
f) shaking the whole blood/polymer particles composition to allow platelets in the whole blood to adhere directly to the surface of the polymer particles;
g) measuring the number of platelets in the whole blood/polymer particles composition after shaking that remain in suspension in the composition; and
h) determining the percent level of adhesion of the platelets by comparing the number of platelets in suspension prior to shaking with the number of platelets in suspension after shaking.
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Abstract
The invention relates to measuring of the adhesion of platelets ex vivo. In this method, monodisperse polymer particles are added to blood, and the number of platelets in the blood are measured before and after shaking of the sample. The level of adhesion of the platelets is determined by comparing the number of platelets prior to shaking with the number of platelets after shaking. Methods of establishing an adhesion index and diagnosis of a patient are also provided.
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12 Claims
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1. A diagnostic method for measuring the adhesion of platelets ex vivo comprising
a) selecting an amount and type of monodisperse polymer particles derived from at least one artificial polymer; -
b) providing a sample of whole blood; c) adding said monodisperse polymer particles to the sample of whole blood to form a blood/polymer particles composition; d) measuring the number of platelets in the whole blood/polymer particles composition; f) shaking the whole blood/polymer particles composition to allow platelets in the whole blood to adhere directly to the surface of the polymer particles; g) measuring the number of platelets in the whole blood/polymer particles composition after shaking that remain in suspension in the composition; and h) determining the percent level of adhesion of the platelets by comparing the number of platelets in suspension prior to shaking with the number of platelets in suspension after shaking. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12)
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Specification