Optical detection system for flow cytometry

• US 7,486,387 B2
• Filed: 04/04/2007
• Issued: 02/03/2009
• Est. Priority Date: 06/29/2001
• Status: Expired due to Term

• Current Assignee: Honeywell International Inc.
• Original Assignee: Honeywell International Inc.
• Inventors: Fritz, Bernard S.
• Primary Examiner(s): Punnoose; Roy M
• Application Number:
  US11/696,639
• Publication Number:
  US 20070188737A1
• Time in Patent Office: 671 Days
• Field of Search: 356/73, 356/337
• US Class Current: 356/73
• CPC Class Codes: A61B 5/150022 for capillary blood or interstitial fluid
• CPC Class Codes: A61B 5/150213 Venting means
• CPC Class Codes: A61B 5/150221 Valves
• CPC Class Codes: A61B 5/150343 Collection vessels for collecting blood samples from the skin surface, e.g. test tubes, cuvettes sampling from within body cavities A61B5/150015, A61B5/150045
• CPC Class Codes: G01N 15/1456 without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals
• CPC Class Codes: G01N 15/1459 the analysis being performed on a sample stream
• CPC Class Codes: G01N 15/1484 microstructural devices
• CPC Class Codes: G01N 2015/1452 Adjustment of focus; Alignment
• CPC Class Codes: G01N 2015/1486 Counting the particles
• CPC Class Codes: G01N 2015/1497 Particle shape

Abstract

An optical detection system for flow cytometry that uses two or more light sources positioned laterally at different distances from a central axis of a flow stream for providing light through different parts of the flow stream. One or more lenses are used to focus the light from the two or more light sources through the flow stream and onto a common focal point or region on the opposite side of the flow stream. One or more light detectors are then placed at, near or around the common focal point or region. A processor or the like receives at least one output signal from the one or more light detectors to analyze and determine selected characteristics of the flow stream.

Claims

1. An optical detection system for analyzing predetermined characteristics of a flow stream, the flow stream having a central axis along the direction of flow, the optical detection system comprising:

one or more light sources positioned on a first side of the flow stream for providing light through the flow stream;

lens means positioned on the first side of the flow stream for focusing the light from each of the one or more light sources to a common focal point or region located on a second opposite side of the flow stream;

light receiving means for receiving the light from the one or more light sources, and for providing at least one signal in response thereto, at least a portion of the light receiving means located at or near the common focal point or region of the lens means; and

processing means for receiving the at least one signal from the light receiving means and for using the at least one signal for analyzing the predetermined characteristics of the flow stream.

2. The system of claim 1, wherein the light receiving means is an annular shaped detector surrounding the common focal point.

3. The system of claim 2, wherein the annular detector includes two or more annular rings.

4. The system of claim 3, wherein the annular detector includes a central zone and a first annular ring, wherein the first annular ring is adapted to detect forward angle scattering (FALS).

5. The system of claim 4, wherein the annular detector further includes a second annular ring positioned outside the first annular ring, wherein the second annular ring is adapted to detect small angle scattering (SALS).

6. The system of claim 1 having two or more spaced light sources in an array, each light source having a central axis, wherein the lens means includes two or more spaced lenses, each lens positioned relative to a corresponding light source, each lens having a central focal axis that is offset by a distance from its corresponding light source central axis.

7. The system of claim 6, wherein the offset distance between the central focal axis of each lens and its corresponding light source central axis changes across the array such that light rays emitted by each light source is focused onto the common focal point.

8. A method for analyzing predetermined characteristics of a flow stream, the flow stream having a central axis along the direction of flow, the method comprising:

providing light from a first side of the flow stream;

focusing the light to a common focal point or region located on a second opposite side of the flow stream;

receiving the light at or near the common focal point or region; and

determining the predetermined characteristics of the flow stream by analyzing the light that is received at or near the common focal point or region.

9. A method according to claim 8, wherein the light is received at the common focal point or region.

10. A method according to claim 8, wherein the light is received along an annulus that surrounds the common focal point or region.

11. A method according to claim 8, wherein the light is received along two or more annuli that surround the common focal point or region.

12. An optical detection system for analyzing predetermined characteristics of a flow stream, the flow stream having a central axis along the direction of flow, the optical detection system comprising:

first and second arrays of light sources, each light source array including two or more spaced light sources positioned on a first side of the flow stream for providing light through the flow stream;

first and second arrays of lenses, each lens array including two or more lenses, the first and second lens arrays corresponding with the first and second light source arrays, wherein the lenses of the first and second arrays are positioned on the first side of the flow stream for focusing the light from each of the two or more light sources in the first and second light source arrays to first and second common focal points or regions, respectively, located on a second opposite side of the flow stream;

first and second light receiving means for receiving the light from the first and second light source arrays, respectively, and for providing at least one signal in response thereto, at least a portion of the first and second light receiving means located at or near the first and second common focal points or regions of the first and second lens arrays, respectively; and

processing means for receiving the at least one signal from the first and second light receiving means and for using the at least one signal for analyzing the predetermined characteristics of the flow stream.

13. The system of claim 12, wherein the first light receiving means includes an annular shaped detector surrounding the first common focal point, and the second light receiving means includes an annular shaped detector surrounding the second common focal point.

14. The system of claim 13, wherein the first annular detector includes two or more annular rings.

15. The system of claim 14, wherein the first annular detector includes a central zone and a first annular ring, wherein the first annular ring is adapted to detect forward angle scattering (FALS).

16. The system of claim 15, wherein the first annular detector further includes a second annular ring positioned outside the first annular ring, wherein the second annular ring is adapted to detect small angle scattering (SALS).

17. The system of claim 16, wherein the first and second light source arrays, lens arrays, and light receiving means are spaced apart along the central axis of the flow stream by a predetermined distance, and the processing means is configured to determine the velocity of a particle traveling between first and second points along the flow stream.

18. The system of claim 17, wherein the first and second light source arrays are angularly offset relative to the central axis of the flow stream.

19. The system of claim 17, wherein the first and second light source arrays are angularly offset by less than ninety degrees relative to the central axis of the flow stream.

20. The system of claim 12, wherein each light source has a central axis and each lens in the first and second lens arrays is positioned relative to a corresponding light source in the first and second light source arrays, respectively, wherein each lens has a central focal axis that is offset by a distance from its corresponding light source central axis.