System and method for a chemical imaging threat assessor with a probe

• US 7,486,395 B2
• Filed: 10/10/2006
• Issued: 02/03/2009
• Est. Priority Date: 10/07/2005
• Status: Active Grant

• Current Assignee: Chemimage Corporation
• Original Assignee: Chemimage Corporation
• Inventors: Treado, Patrick J., Hutchison, Wesley H., Voigt, Thomas C.
• Primary Examiner(s): Geisel; Kara E
• Application Number:
  US11/544,648
• Publication Number:
  US 20070081156A1
• Time in Patent Office: 847 Days
• Field of Search: 356/301, 356317-318, 356/72, 356/326
• US Class Current: 356/301
• CPC Class Codes: G01J 3/02 Details
• CPC Class Codes: G01J 3/0205 Optical elements not provided otherwise, e.g. optical manifolds, diffusers, windows
• CPC Class Codes: G01J 3/0218 using optical fibers
• CPC Class Codes: G01J 3/0224 using polarising or depolarising elements
• CPC Class Codes: G01J 3/0227 using notch filters
• CPC Class Codes: G01J 3/0256 Compact construction
• CPC Class Codes: G01J 3/0264 Electrical interface; User interface
• CPC Class Codes: G01J 3/027 Control of working procedures of a spectrometer; Failure detection; Bandwidth calculation
• CPC Class Codes: G01J 3/0272 Handheld
• CPC Class Codes: G01J 3/0283 using a charging unit
• CPC Class Codes: G01J 3/0286 Constructional arrangements for compensating for fluctuations caused by temperature, humidity or pressure, or using cooling or temperature stabilization of parts of the device; Controlling the atmosphere inside a spectrometer, e.g. vacuum
• CPC Class Codes: G01J 3/0289 Field-of-view determination; Aiming or pointing of a spectrometer; Adjusting alignment; Encoding angular position; Size of measurement area; Position tracking
• CPC Class Codes: G01J 3/0291 Housings; Spectrometer accessories; Spatial arrangement of elements, e.g. folded path arrangements
• CPC Class Codes: G01J 3/10 Arrangements of light sources specially adapted for spectrometry or colorimetry
• CPC Class Codes: G01J 3/26 using multiple reflection, e.g. Fabry-Perot interferometer, variable interference filters
• CPC Class Codes: G01J 3/2803 using photoelectric array detector
• CPC Class Codes: G01J 3/2823 Imaging spectrometer
• CPC Class Codes: G01J 3/44 Raman spectrometry; Scattering spectrometry ; Fluorescence spectrometry
• CPC Class Codes: G01J 3/4406 Fluorescence spectrometry
• CPC Class Codes: G01J 3/447 Polarisation spectrometry
• CPC Class Codes: G01N 2021/656 : Raman microprobe
• CPC Class Codes: G01N 21/65 : Raman scattering
• CPC Class Codes: G01N 2201/0846 : Fibre interface with sample, e.g. for spatial resolution

Abstract

The disclosure relates to a portable and/or handheld bioagent detector and methodology described herein that is based in part on advanced Raman Chemical Imaging (“RCI”) technology. According to one embodiment of the present disclosure, the detection system may include a fiber array spectral translator (“FAST”) and may also include a probe which may include a complementary metal oxide semiconductor (CMOS) camera. The probe alleviates the need to place the main instrument close to an unconfined release of a potentially hazardous material and facilitates analysis of a sample that is situated in a hard-to-reach location while minimizing contamination of the detector and operator.

Claims

1. A portable system for obtaining a spectrum of a sample, comprising:

an instrument module comprising a photon source, a spectrometer, and a photon detector;

a handheld probe operably connected to said instrument module and independently movable with respect to said instrument module, said probe comprising a complementary metal oxide semiconductor (CMOS) camera contained substantially within a housing, wherein said housing includes a positioning guide for spacing apart said handheld probe from said sample;

said photon source for providing a first plurality of photons to interact with said sample to thereby form a second plurality of photons;

an optical lens contained substantially within said housing for collecting said second plurality of photons;

a filter for blocking a portion of said first plurality of photons present in an optical path with said second plurality of photons;

a fiber optic array for directing said second plurality of photons passing through said filter to said spectrometer, wherein said fiber optic array is a fiber array spectral translator and optical fibers comprising said fiber optic array are arranged in a two-dimensional array at a first end and a one-dimensional array at a second end of the fiber optic array; and

said photon detector receiving said second plurality of photons having passed through said spectrometer to thereby obtain a spectrum of said sample,

wherein said sample includes at least one biothreat agent selected from the group consisting of bacterial spores, live cells, viruses and toxins, and at least one material or chemical present in the normal ambient environment from which said sample is obtained.

2. The system of claim 1 wherein said handheld probe further comprises a light shield.

3. The system of claim 1 wherein said CMOS camera takes an image of said sample.

4. The system of claim 1 wherein said photon detector is a charge-coupled device.

5. The system of claim 1 wherein said spectrometer is a diffusion grating.

6. The system of claim 1 further comprising a microprocessor for analyzing an output signal from said photon detector.

7. The system of claim 1 further comprising a memory device for storing a spectrum of a biothreat agent.

8. The system of claim 7 further comprising a microprocessor which compares an output signal from said photon detector with the stored spectrum in said memory device.

9. The system of claim 8 further comprising a display unit which displays information based on said comparison.

10. The system of claim 9 further comprising a device for accepting input from a user.

11. The system of claim 1 wherein said first plurality of photons has a wavelength approximately equal to 532 nanometers.

12. The system of claim 1 wherein said first plurality of photons has a wavelength between 522 and 542 nanometers.

13. The system of claim 1 wherein said first plurality of photons has a wavelength in a range of wavelengths consisting of white light, near infrared light, infrared light, and ultraviolet light.

14. The system of claim 1 wherein said second plurality of photons comprises photons scattered by said sample.

15. The system of claim 14 wherein said scattered photons are Raman scattered photons.

16. The system of claim 1 wherein said second plurality of photons comprises photons emitted by said sample.

17. The system of claim 1 wherein said second plurality of photons comprises photons reflected by said sample.

18. A handheld portable system for obtaining a spectrum of a sample, comprising:

an instrument module comprising a photon source, a spectrometer, and a photon detector;

a probe operably connected to said instrument module and independently movable with respect to said instrument module, said probe comprising a complementary metal oxide semiconductor (CMOS) camera contained substantially within a housing, wherein said housing includes a positioning guide for spacing apart said probe from said sample;

said photon source for providing a first plurality of photons to interact with said sample to thereby form a second plurality of photons;

an optical lens for collecting said second plurality of photons;

a filter for blocking a portion of said first plurality of photons present in an optical path with said second plurality of photons;

a fiber optic array for directing said second photons passing through said filter to said spectrometer, wherein optical fibers comprising the fiber optic array are arranged in a two-dimensional array at a first end and a one-dimensional array at a second end of the fiber optic array, and wherein said first end accepts said second plurality of photons passing through said filter and said second end directs said second plurality of photons to said spectrometer;

said photon detector receiving said second plurality of photons having passed through said spectrometer to thereby obtain a spectrum of said sample;

a memory device for storing a spectrum of a biothreat agent;

a microprocessor for comparing an output signal from said photon detector with the stored spectrum in said memory device; and

a display unit for displaying information based on said comparison,

wherein said sample includes at least one biothreat agent selected from the group consisting of bacterial spores, live cells, viruses and toxins, and at least one material or chemical present in the normal ambient environment from which said sample is obtained.

19. A method for obtaining a spectrum of a sample, comprising:

(a) providing a portable instrument module comprising a photon source, a spectrometer, and a photon detector;

(b) placing a handheld probe near the sample wherein said probe is operably connected to said instrument module and independently movable with respect to said instrument module, said probe comprising a complementary metal oxide semiconductor (CMOS) camera contained substantially within a housing, wherein said housing includes a positioning guide for spacing apart said handheld probe from said sample;

(c) illuminating said sample with a first plurality of photons to interact with said sample to thereby form a second plurality of photons, said fast plurality of photons passing through said probe;

(d) collecting said second plurality of photons;

(e) blocking a portion of said fast plurality of photons present in an optical path with said second plurality of photons; and

(f) directing said second plurality of photons to said spectrometer and then to said photon detector to thereby obtain a spectrum of said sample,

wherein said sample includes at least one biothreat agent selected from the group consisting of bacterial spores, live cells, viruses and toxins, and at least one material or chemical present in the normal ambient environment from which said sample is obtained.

20. The method of claim 19 further comprising:

(g) storing a predetermined spectrum of a biothreat agent;

(h) comparing an output signal from said photon detector with the stored spectrum; and

(i) displaying information based on said comparison.

21. The method of claim 19 wherein said fast plurality of photons has a wavelength approximately equal to 532 nanometers.

22. The method of claim 19 wherein said first plurality of photons has a wavelength in a range of wavelengths consisting of white light, near infrared light, infrared light, and ultraviolet light.

23. The method of claim 19 wherein said second plurality of photons comprises photons scattered by said sample.

24. The method of claim 23 wherein said scattered photons are Raman scattered photons.

25. The method of claim 19 wherein said second plurality of photons comprises photons emitted by said sample.

26. The method of claim 19 wherein said second plurality of photons comprises photons reflected by said sample.