Electronically readable microarray with electronic addressing function
First Claim
Patent Images
1. A method of detecting a target in a sample, the method comprising:
- (a) contacting a microarray with the sample, the microarray comprising a plurality of features disposed on a substrate, each feature comprising a first electrode disposed on the substrate, a second electrode disposed on the substrate, a pad of resistive material disposed on the substrate between the first electrode and the second electrode, said pad of material comprising a material selected from the group consisting of carbon thin film, metal thin film, metal nitride, nichrom (NiCr), tantalum nitride (Ta2N), silicon chrome, and metal oxide, and a probe supported on the pad of resistive material, the substrate comprising integrated addressing circuitry in operable relation to each of the plurality of features;
(b) applying a source of metal ions to the plurality of features to result in metal deposited between the first electrode and the second electrode of said features where the target is present, said metal providing a change in an observable property of at least one of the plurality of features;
(c) providing a signal to the addressing circuitry to select one of the plurality of features to be interrogated; and
(d) measuring the observable property at the selected feature to detect the target.
1 Assignment
0 Petitions
Accused Products
Abstract
Methods and apparatus for electronically addressing and interrogating microarrays are disclosed. The described microarrays include a plurality of features disposed on a substrate. Each of the features has a first electrode disposed on the substrate, a second electrode disposed on the substrate, and a probe disposed between the first electrode and second electrode. The substrate also includes addressing circuitry in operable relation to the features. Method of using the microarrays are also described.
-
Citations
19 Claims
-
1. A method of detecting a target in a sample, the method comprising:
-
(a) contacting a microarray with the sample, the microarray comprising a plurality of features disposed on a substrate, each feature comprising a first electrode disposed on the substrate, a second electrode disposed on the substrate, a pad of resistive material disposed on the substrate between the first electrode and the second electrode, said pad of material comprising a material selected from the group consisting of carbon thin film, metal thin film, metal nitride, nichrom (NiCr), tantalum nitride (Ta2N), silicon chrome, and metal oxide, and a probe supported on the pad of resistive material, the substrate comprising integrated addressing circuitry in operable relation to each of the plurality of features; (b) applying a source of metal ions to the plurality of features to result in metal deposited between the first electrode and the second electrode of said features where the target is present, said metal providing a change in an observable property of at least one of the plurality of features; (c) providing a signal to the addressing circuitry to select one of the plurality of features to be interrogated; and (d) measuring the observable property at the selected feature to detect the target. - View Dependent Claims (2, 3, 4, 5, 6, 7, 15)
-
- 8. A microarray comprising a plurality of features disposed on a substrate, each feature comprising a first electrode disposed on the substrate, a second electrode disposed on the substrate, a pad of resistive material disposed on the substrate between the first electrode and the second electrode, and a probe supported on the pad of resistive material, the substrate comprising integrated addressing circuitry in operable relation to the features, the addressing circuitry operable to select a given feature to allow interrogation of the selected feature, and the pad of resistive material comprising a material selected from the group consisting of carbon thin film, metal thin film, metal nitride, nichrom (NiCr), tantalum nitride (Ta2N), silicon chrome, and metal oxide.
- 16. A microarray comprising a plurality of features disposed on a substrate, each feature comprising a first electrode disposed on the substrate, a second electrode disposed on the substrate, a pad of resistive material disposed on the substrate between the first electrode and the second electrode, and a probe supported on the pad of resistive material, the substrate comprising integrated addressing circuitry in operable relation to the features, the addressing circuitry operable to select a given feature to allow interrogation of the selected feature, and the pad of resistive material comprising a plurality of segments with fissures between the segments.
Specification