Device for implementing an analysis pack, analysis pack and method using same

• US 7,491,497 B2
• Filed: 10/18/2005
• Issued: 02/17/2009
• Est. Priority Date: 06/22/1999
• Status: Expired due to Fees

• Current Assignee: Biomerieux SA (Compagnie Merieux Alliance SAS)
• Original Assignee: Biomerieux SA (Compagnie Merieux Alliance SAS)
• Inventors: Privat, Marie, Paris, Cécile, Colin, Bruno, Dachaud, Jacques
• Primary Examiner(s): Kim; Young J
• Application Number:
  US11/251,758
• Publication Number:
  US 20060078466A1
• Time in Patent Office: 1,218 Days
• Field of Search: None
• US Class Current: 435/6.12
• CPC Class Codes: B01L 3/5025 for parallel transport of multiple samples
• CPC Class Codes: G01N 35/1097 characterised by the valves valves in general F16K

Abstract

A device for implementing a test card inside which sequential reactions and fluid transfer operations are performed under the effect of a control integrated into the card; the card includes at least two sequential reaction systems arranged in parallel, each reaction system having at least two fluid transfer systems arranged in series. A test card and the method for implementing the device and the card are also disclosed. The device contains at least one actuator per reaction line, and all the actuators are at a regular distance from one another, with the actuators preferably being equidistant. The invention is particularly applicable for the micromanipulation of fluids in biological applications.

Claims

1. A test card inside which sequential reactions and fluid transfer operations are performed under the effect of control means integrated into the card, said card comprising:

at least two sequential reaction lines arranged in parallel, each reaction line comprising at least two fluid transfer systems arranged in series, at least two valves per parallel reaction line, the valves making it possible to control fluid transfers in series within a channel from an initial level (A, B or C) to a subsequent level (B, C or D), each level corresponding to at least one processing, each valve being made up of at least a means which can be deformed by an actuator for directly or indirectly closing of said channel, wherein a distance d₁ between two counterpart valves belonging to different reaction lines is constant, whatever the initial level (A, B or C) from which fluid transfers which are controlled by said counterpart valves are performed, and a distance d₂ between the two valves belonging to the same reaction line is constant from one reaction line to another.

2. The card of claim 1, wherein each reaction line includes at least one initial compartment, one receiving compartment, a fluid-carrying channel linking the two compartments, the valve positioned on said fluid-carrying channel acting on said channel as a control means of the fluid flow generated by a transfer means, and wherein the arrangement of the valves on the test card is such that the fluids in each reaction line are allowed to flow between the initial compartment in each reaction line and the receiving compartment in each reaction line at the same time.

3. The test card of claim 2, wherein the valves are arranged along a straight line with all the valves being equidistant from one another.

4. The test card of claim 3, wherein the straight line is perpendicular to one side of the test card.

5. The test card of claim 2, wherein at least one of the compartments is associated with at least one buffer supply, and the buffer supply is located on the opposite side of the test card in relation to the compartment with which it is associated.

6. The test card of claim 1, wherein each compartment which contains at least one reagent which is to be brought into contact with the sample or an aliquot of the sample, contains a means for keeping a tablet in position, with said means at a distance from a bottom of said compartment such that said tablet is not in contact with said bottom of said compartment.

7. The test card of claim 6, wherein at the center of all or part of compartments there are a plurality of reinforcing stanchions, which make up holding means that form an isosceles triangle.

8. The test card of claim 7, wherein these stanchions are adapted to hold in place a tablet which contains particles.

9. The test card of claim 8, wherein said tablet contains magnetic particles.

10. The test card of claim 7, wherein said plurality of reinforcing stanchions comprises three stanchions.

11. The test card of claim 6, wherein said tablet comprises an agglomerate of the reagent(s).

12. The test card of claim 1, wherein said means which can be deformed by an actuator comprises a flexible film covering at least a part of the upper and/or lower part of the test card.

13. The test card of claim 1, wherein each level has a fluid inlet and a separate fluid outlet.

14. A device for implementing the test card of claim 1, which comprises at least one actuator per reaction line of said test card of claim 1 and in which all actuators are at a constant distance from one another, the device comprising means for moving the test card forward in relation to the actuators, and/or comprising means for moving said actuators forward in relation to said card, and in which each actuator can be activated independently of the others.

15. The device of claim 14, wherein the action of the actuators on the card is at a perpendicular angle:

in relation to the surface of said card where the actuators operate, and/or in relation to a direction of movements of both the card and actuator.

16. The device of claim 14, wherein all the actuators are mounted on a single ramp, and the ramp is rectilinear, perpendicular to the direction of movements of the actuators, and/or perpendicular to the direction of movements of the card and/or of the device with respect to the card.

17. The device of claim 14, wherein actuators in the same ramp are equidistant.

18. A method for implementing the test card of claim 1, comprising the following steps:

generating a pressure differential inside said test card with respect to the outside, introducing at least one sample to be tested into said test card, moving each sample or aliquot of sample within the test card for testing purposes, and withdrawing from or keeping in said test card a part of each introduced sample.

19. The method of claim 18, wherein the method includes the following steps:

denaturing DNA and/or RNA in each sample or aliquot of sample being tested in a compartment of the test card, immobilizing DNA and/or RNA molecules in each sample or aliquot of sample being tested on magnetic beads contained within the test card, amplifying said DNA and/or RNA in at least one compartment of the test card, and screening of each sample or aliquot of sample in a compartment of the test card to check whether amplification has occurred.

20. The method of claim 19, wherein the method includes a preliminary extracting step in the test card corresponding to lysis of any cells contained in the sample.

21. The method of claim 20, wherein the method includes the step of purifying the sample in the test card after extraction and before amplification.

22. The method of claim 18, wherein the method includes the step of analyzing transcripts in the test card by hybridization using a biochip following amplification.

23. The method of claim 18, wherein the test card is used in an inclined or a vertical position during testing.

24. The method of claim 18, wherein the test card is displaced during the various different steps and/or operations in a sequential manner.

25. The method of claim 24, wherein the test card is displaced along two perpendicular axes in order to align the valves with the valve actuators.

26. The method of claim 18, wherein the step of generating a pressure differential inside said test card also generates a depression.