Apparatus and method for detection with a scanning microscope
First Claim
1. A method for detection with a scanning microscope, the scanning microscope comprising a scanning device (7) that guides an illuminating light beam (3) through a scanning optical system (12) and a microscope optical system (13) and over or through a specimen (15), the specimen (15) being illuminated in multiple pixels (Px,y) with the illuminating light beam (3), and the light emanating from the specimen (15) being received with a detector unit (19), and detected signals being generated, wherein the scanning microscope further comprises a digital circuit comprising a summing element and a dividing element,the method comprising the following steps:
- interrogating a detected signal of the detector unit (19) by means of said digital circuit;
summing the detected signal of the detector unit (19) by means of said summing element resulting in a sum of detected signals;
repeating said steps of interrogating and summing for a number of detected signals, wherein said number of detected signals defines in a cycle;
dividing the sum of detected signals by the number of detected signals by means of said dividing element resulting in an average detected signal;
interrogating a detected signal of the detector unit (19) by means of said digital circuit;
setting said summing element equal to said detected signal of the detector unit (19); and
,repeating the steps above.
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Abstract
An apparatus and a method for detection with a scanning microscope (1) are disclosed. The scanning microscope (1) encompasses a scanning device (7) that guides an illuminating light beam (3) through a scanning optical system (12) and a microscope optical system (13) and over or through a specimen (15). A digital circuit (30), which periodically interrogates the detected signals within a pixel (Px,y) and calculates an average therefrom, is placed after the detector unit (19).
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Citations
30 Claims
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1. A method for detection with a scanning microscope, the scanning microscope comprising a scanning device (7) that guides an illuminating light beam (3) through a scanning optical system (12) and a microscope optical system (13) and over or through a specimen (15), the specimen (15) being illuminated in multiple pixels (Px,y) with the illuminating light beam (3), and the light emanating from the specimen (15) being received with a detector unit (19), and detected signals being generated, wherein the scanning microscope further comprises a digital circuit comprising a summing element and a dividing element,
the method comprising the following steps: -
interrogating a detected signal of the detector unit (19) by means of said digital circuit; summing the detected signal of the detector unit (19) by means of said summing element resulting in a sum of detected signals; repeating said steps of interrogating and summing for a number of detected signals, wherein said number of detected signals defines in a cycle; dividing the sum of detected signals by the number of detected signals by means of said dividing element resulting in an average detected signal; interrogating a detected signal of the detector unit (19) by means of said digital circuit; setting said summing element equal to said detected signal of the detector unit (19); and
,repeating the steps above. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30)
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Specification