Detection of biochemical interactions on a biosensor using tunable filters and tunable lasers

• US 7,497,992 B2
• Filed: 05/08/2003
• Issued: 03/03/2009
• Est. Priority Date: 05/08/2003
• Status: Expired due to Fees

• Current Assignee: X-Body Incorporated (Bristol-Myers Squibb Company), Regents of the University of California (University of California)
• Original Assignee: Sru Biosystems Incorporated, Regents of the University of California (University of California)
• Inventors: Cunningham, Brian T., Mateus, Carlos, Chang-Hasnain, Constance, Li, Peter Y.
• Primary Examiner(s): Warden; Jill
• Assistant Examiner(s): TURK, NEIL N
• Application Number:
  US10/434,015
• Publication Number:
  US 20040223881A1
• Time in Patent Office: 2,126 Days
• Field of Search: 385/12, 370/20, 422/82.11, 356/326
• US Class Current: 422/82.05
• CPC Class Codes: G01N 21/39 using tunable lasers
• CPC Class Codes: G01N 21/45 using interferometric methods; using Schlieren methods
• CPC Class Codes: G01N 21/75 Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated systems in which material is burnt in a flame or plasma G01N21/72, G01N21/73
• CPC Class Codes: G01N 21/7743 the reagent-coated grating coupling light in or out of the waveguide
• CPC Class Codes: G01N 33/54373 involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings

Abstract

An apparatus and method for detection of peak wavelength values of colorimetric resonant optical biosensors using tunable filters and tunable lasers is provided. Biomolecular interactions may be detected on a biosensor by directing collimated white light towards a surface of the biosensor. Molecular binding on the surface of the biosensor is indicated by a shift in the peak wavelength value of reflected or transmitted light from the biosensor, while an increase in the wavelength corresponds to an increase in molecular absorption. A tunable laser light source may generate the collimated white light and a tunable filter may receive the reflected or transmitted light and pass the light to a photodiode sensor. The photodiode sensor then quantifies an amount of the light reflected or transmitted through the tunable filter as a function of the tuning voltage of the tunable filter.

Claims

1. A measuring apparatus for use in detecting a biochemical interaction, the measuring apparatus comprising, in combination:

a biosensor, wherein the biosensor comprises a colorimetric resonant optical biosensor embedded into a surface of a device receiving a sample to be tested,

an illumination system including a light source for generating collimated light, the illumination system and directing the collimated light towards a surface of the biosensor;

a tunable filter operable to receive light transmitted through the biosensor and to pass a narrow band of light having wavelengths substantially centered at a passband wavelength and to reflect substantially all other wavelength, wherein the passband wavelength is adjusted according to a tuning voltage of the tunable filter, and

a detector receiving light from the tunable filter wherein the detector of the measuring apparatus detects a biochemical interaction occurring on the colormetric resonant optical biosensor.

2. The invention of claim 1, wherein the tunable filter comprises a micro-electro-mechanical (MEM) cantilever device.

3. The invention of claim 1, wherein the light source comprises a broadband light source.

4. The invention of claim 1, wherein the light source comprises an LED.

5. The invention of claim 1, wherein the light source comprises a tunable laser.

6. The invention of claim 5, wherein the tunable laser comprises a vertical cavity surface emitting laser (VCSEL).

7. The invention of claim 1, wherein the passband wavelength is adjustable over a wavelength range that includes a wavelength at which said biosensor exhibits an optical resonance mode.

8. The invention of claim 1, wherein the tuning voltage is adjusted as a function of time, so as to sweep the passband wavelength.

9. The invention of claim 1, further comprising a photodiode sensor positioned to receive the narrow band of light having wavelengths substantially centered at the passband wavelength from the tunable filter.

10. invention of claim 9, wherein the tunable filter includes the photodiode sensor.

11. invention of claim 9, wherein an output of the photodiode sensor quantifies an amount of the light transmitted through the tunable filter as a function of the tuning voltage of the tunable filter.

12. The invention of claim 1, further comprising a plurality of tunable filters, wherein each tunable filter passes light transmitted through a respective region of the bio sensor.

13. invention of claim 12, wherein the plurality of tunable filters are arranged in a linear array.

14. Apparatus comprising in combination:

a colorimetric resonant optical biosensor embedded in the surface of a device for receiving a sample;

a measuring apparatus for use in detecting a wavelength of maximum intensity in reflected light reflected from the biosensor, the measuring apparatus comprising;

(1) a light source for generating collimated white light;

(2) a power splitter receiving the collimated while light from the light source and directing the collimated white light towards a surface of the biosensor; and

(3) a tunable detector that receives light reflected by the biosensor and measures a radiation spectrum of the light reflected from the biosensor, wherein the tunable detector includes a filter which passes a narrow band of light having wavelengths substantially centered at a passband wavelength from the light reflected by the biosensor, where the passband wavelength is adjusted according to a tuning voltage of the tunable detector;

wherein the tunable detector of the measuring apparatus detects a biochemical interaction occurring on the colorimetric resonant optical biosensor.

15. invention of claim 14, wherein the tunable detector has a micro-electro-mechanical (MEM) cantilever device.

16. The invention of claim 14, wherein the light source comprises a broadband light source.

17. The invention of claim 14, wherein the light source comprises an LED.

18. The invention of claim 14, further comprising an optical fiber probe assembly positioned in an optical path between the power splitter and the biosensor having a first fiber positioned to receive the collimated white light from the power splitter and to direct the collimated white light towards the surface of the biosensor.

19. The invention of claim 18, wherein the optical fiber probe assembly includes a second fiber for receiving the light reflected by the biosensor and directing the light reflected by the biosensor to the tunable detector.

20. The invention of claim 14, further comprising a collimation device positioned to receive the collimated white light from the power splitter and to direct the collimated white light towards the surface of the biosensor.