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Polymerases for analyzing or typing polymorphic nucleic acid fragments and uses thereof

  • US 7,501,237 B2
  • Filed: 06/27/2001
  • Issued: 03/10/2009
  • Est. Priority Date: 02/07/1997
  • Status: Expired due to Fees
First Claim
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1. A method of identifying, analyzing or typing a polymorphic DNA fragment in a sample of DNA, said method comprising:

  • (a) contacting said sample of DNA with one or more DNA polymerases, wherein said one or more DNA polymerases comprise one or more mutations or modifications in the O-helix of said one or more DNA polymerases which reduce their ability to add one or more non-templated nucleotides to the 3′

    terminus of a synthesized DNA molecule;

    (b) amplifying said polymorphic DNA fragment within said sample to produce a population of amplified DNA fragments, wherein less than about 50% of said amplified DNA fragments have one or more non-templated 3′

    nucleotides compared to amplification products produced by Taq DNA polymerase assayed under the same conditions; and

    (c) analyzing said amplified polymorphic DNA fragment.

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