Process for producing polypeptide
First Claim
1. A process for producing a polypeptide which comprises:
- (a) selecting a rat cell line capable of adapting in a serum-free medium(b) culturing the rat cell line selected in step (a) in a serum-free medium while an insulin concentration in the culture is maintained at 10 mg/l or above, followed by culturing while an insulin concentration in the culture is maintained at 20 mg/l or below, and(c) recovering the desired polypeptide from the culture,wherein the step (a) is carried out by inoculating a rat cell line into a serum-free medium at the cell density of 1×
105 to 10×
105 cells/ml of serum free medium and selecting a rat cell line that reaches a cell density of 10×
105 to 40×
105 cells/ml of serum-free medium.
3 Assignments
0 Petitions
Accused Products
Abstract
The present invention relates to a process for producing a desired polypeptide using rat cells. Specifically, the present invention relates to a process for producing the polypeptide which comprises culturing rat cells such as YB2/3HL.P2.G11.16Ag.20 (hereinafter referred to as YB2/0), preferably rat cells to which a recombinant DNA comprising DNA encoding a desired polypeptide such as an immunologically functional molecule is introduced, in a medium which does not contain serum (hereinafter referred to as a serum-free medium). Among the desired polypeptides obtained by the process of the present invention, an antibody obtained by using a transformant of YB2/0 has a high antibody-dependent cell-mediated cytotoxic activity (hereinafter sometimes referred to as ADCC activity) and is useful as a pharmaceutical agent.
110 Citations
18 Claims
-
1. A process for producing a polypeptide which comprises:
-
(a) selecting a rat cell line capable of adapting in a serum-free medium (b) culturing the rat cell line selected in step (a) in a serum-free medium while an insulin concentration in the culture is maintained at 10 mg/l or above, followed by culturing while an insulin concentration in the culture is maintained at 20 mg/l or below, and (c) recovering the desired polypeptide from the culture, wherein the step (a) is carried out by inoculating a rat cell line into a serum-free medium at the cell density of 1×
105 to 10×
105 cells/ml of serum free medium and selecting a rat cell line that reaches a cell density of 10×
105 to 40×
105 cells/ml of serum-free medium. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18)
-
Specification