×

Process for producing polypeptide

  • US 7,504,256 B1
  • Filed: 10/19/2000
  • Issued: 03/17/2009
  • Est. Priority Date: 10/19/1999
  • Status: Active Grant
First Claim
Patent Images

1. A process for producing a polypeptide which comprises:

  • (a) selecting a rat cell line capable of adapting in a serum-free medium(b) culturing the rat cell line selected in step (a) in a serum-free medium while an insulin concentration in the culture is maintained at 10 mg/l or above, followed by culturing while an insulin concentration in the culture is maintained at 20 mg/l or below, and(c) recovering the desired polypeptide from the culture,wherein the step (a) is carried out by inoculating a rat cell line into a serum-free medium at the cell density of 1×

    105 to 10×

    105 cells/ml of serum free medium and selecting a rat cell line that reaches a cell density of 10×

    105 to 40×

    105 cells/ml of serum-free medium.

View all claims
  • 3 Assignments
Timeline View
Assignment View
    ×
    ×