Method of manufacturing probe carrier

• US 7,507,576 B2
• Filed: 01/19/2007
• Issued: 03/24/2009
• Est. Priority Date: 03/28/2001
• Status: Expired due to Fees

• Current Assignee: Canon Kabushiki Kaisha (Canon Inc.)
• Original Assignee: Canon Kabushiki Kaisha (Canon Inc.)
• Inventors: Mihashi, Naoto, Yamamoto, Nobuko
• Primary Examiner(s): Sisson; Bradley L
• Application Number:
  US11/624,980
• Publication Number:
  US 20070117139A1
• Time in Patent Office: 795 Days
• Field of Search: None
• US Class Current: 435/287.2
• CPC Class Codes: B01J 19/0046 Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays synthesis methods per se C40B50/00
• CPC Class Codes: B01J 2219/00317 Microwell devices, i.e. having large numbers of wells
• CPC Class Codes: B01J 2219/00421 using centrifugation
• CPC Class Codes: B01J 2219/00605 the compounds being directly bound or immobilised to solid supports
• CPC Class Codes: B01J 2219/00621 by physical means, e.g. trenches, raised areas
• CPC Class Codes: B01J 2219/00673 Slice arrays
• CPC Class Codes: B01J 2219/00677 Ex-situ synthesis followed by deposition on the substrate
• CPC Class Codes: B01J 2219/00722 Nucleotides
• CPC Class Codes: B01L 3/5085 for multiple samples, e.g. microtitration plates
• CPC Class Codes: B82Y 30/00 Nanotechnology for materials or surface science, e.g. nanocomposites
• CPC Class Codes: C40B 40/06 Libraries containing nucleotides or polynucleotides, or derivatives thereof
• CPC Class Codes: C40B 60/14 for creating libraries
• CPC Class Codes: Y10T 156/1052 with cutting, punching, tearing or severing
• CPC Class Codes: Y10T 436/143333 Saccharide [e.g., DNA, etc.]

Abstract

A flat plate-shaped probe carrier for carrying probes such as single stranded DNAs, proteins, etc., which are reactive specifically with target substances comprises a plurality of ring bodies arranged substantially in parallel and the external space among the ring bodies is filled with a filler such that the openings of the ring bodies are oriented to the surface of the probe carrier. Each ring body has a region for fixing a probe adapted to be bonded specifically to a target substance on its inner wall. The probe carrier is produced by bundling a plurality of hollow tubular members in parallel, then filling the external space among the bundled hollow members with a filler, and cutting the bundle along a plane intersecting the axial direction of the tubular members. Probes are fixed to the fixing region before or after filling the external space.

Claims

1. A method of manufacturing a probe carrier comprising a plurality of ring bodies, wherein the ring bodies have an internal surface, and the internal surface has a region for fixing a probe adapted to be bonded specifically to a target substance, said method comprising:

a step of bundling a plurality of hollow tubular members to arrange them substantially in parallel with each other;

a step of filling an external space among the bundled hollow tubular members with a filler and solidifying the filler;

a step of cutting the bundle of the tubular members filled with the filler along a plane intersecting an axial direction of the tubular members to produce a flat plate-shaped carrier comprising a plurality of ring bodies having respective internal openings;

a step of sealing the tubular members at one end before filling the space with the filler; and

a step of fixing probes to respective regions for fixing a probe before or after filling the space with the filler,

wherein the filler is in a gel state before the step of filling the external space, and the external space is filled with the filler by applying a centrifugal force to the filler making it flow in a sol state, and

wherein solidification is completed as the application of the centrifugal force is reduced, but while gyration still takes place.

2. The method according to claim 1, wherein the target substance is a nucleic acid and the probe has a sequence complementary relative to part or all of the base sequence of the nucleic acid and is a single-stranded nucleic acid adapted to be specifically hybridized with the nucleic acid.

3. A method for forming a flat plate-shaped probe carrier comprising the steps of:

providing a plurality of tubular members having an inner surface and an outer surface and being sealed at one end;

filling an external space among the tubular members with a filler by applying a centrifugal force to the filler so that solidification of the filler is completed as the application of the centrifugal force is reduced, but while gyration still takes place;

fixing a probe on the inner surface of the tubular members before or after filling the external space with the filler; and

forming the flat plate-shaped probe carrier comprising ring bodies by cutting the tubular members.

4. The method according to claim 3, wherein the ring bodies comprise fiber, glass or resin.

5. The method according to claim 3, wherein the ring bodies comprise a polypropylene film, polyethylene film or cupro-ammonia cellulose regenerated film.

6. The method according to claim 3, wherein the filler comprises polyurethane.

7. The method according to claim 3, wherein the external space is filled so that substantially no said external space is left.

8. The method according to claim 3, wherein the flat plate-shaped probe carrier consists of the bundle of hollow tubes, probes and the filler.

9. The method according to claim 3, wherein the probe is a single-stranded nucleic acid.