Compositions and methods for detecting certain Flaviviruses, including members of the Japanese encephalitis virus serogroup
First Claim
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1. A kit for the detection of a nucleic acid of a member of the Japanese encephalitis virus serogroup, comprising:
- a) a first oligonucleotide that comprises SEQ ID NO.;
4;
b) a second oligonucleotide that comprises SEQ ID NO;
12; and
c) a detectably-labeled third oligonucleotide that comprises SEQ ID NO;
20,wherein the first and second oligonucleotide lengths are from 25 to 100 nucleotides, and the third oligonucleotide length is from 28 to 100 nucleotides.
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Abstract
The present invention provides rapid and accurate methods, primers, probes and kits for identifying the presence of a certain flaviviruses in a sample. Flaviviruses that can be detected include members of the Japanese encephalitis virus serogroup, Dengue virus, St Louis encephalitis virus, Montana myotis leukoencephalitis virus, Modoc virus, and Yellow Fever virus. The primers and probes of the invention can hybridize to regions in the 3′ untranslated region of the viral genomes to be detected.
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27 Claims
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1. A kit for the detection of a nucleic acid of a member of the Japanese encephalitis virus serogroup, comprising:
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a) a first oligonucleotide that comprises SEQ ID NO.;
4;b) a second oligonucleotide that comprises SEQ ID NO;
12; andc) a detectably-labeled third oligonucleotide that comprises SEQ ID NO;
20,wherein the first and second oligonucleotide lengths are from 25 to 100 nucleotides, and the third oligonucleotide length is from 28 to 100 nucleotides. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27)
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Specification