Device and method for rapid tissue preparations for histological investigations

• US 7,514,042 B2
• Filed: 10/02/2002
• Issued: 04/07/2009
• Est. Priority Date: 10/06/2001
• Status: Expired due to Fees

• Current Assignee: Leica Microsystems Gmbh (Danaher Corporation)
• Original Assignee: Leica Microsystems Gmbh (Danaher Corporation)
• Inventors: Pauliny, Ferdinand, Goll, Hubert, Lihl, Reinhard
• Primary Examiner(s): Griffin; Walter D
• Assistant Examiner(s): Ramdhanie; Bobby
• Application Number:
  US10/491,386
• Publication Number:
  US 20050118670A1
• Time in Patent Office: 2,379 Days
• Field of Search: 422/99
• US Class Current: 422/536
• CPC Class Codes: G01N 1/31 Apparatus therefor
• CPC Class Codes: G01N 1/44 Sample treatment involving radiation, e.g. heat
• CPC Class Codes: G01N 2001/364 using resins, epoxy

Abstract

The device (1) for fast tissue processing for histological examination consists of a housing (2) that is covered by a transparent hood (70). A baseplate (3) divides the device (1) into an upper part (4a) and a lower part (4b). Several process chambers that are served by a transport mechanism (6) with holders (40) for tissue specimens are provided for on the baseplate (3). At least one reservoir (74) is provided for in the lower part (4b) of the device, which has a flow connection to at least one process chamber. An operating panel (76) allows the user to enter the appropriate data.

Claims

1. A device for processing tissue samples for histological examination, comprising:

a housing comprising an upper part and a lower part divided by a baseplate;

several process chambers, each process chamber comprising a side wall and a floor, the process chambers being arranged on the baseplate and capable of being filled with a reagent;

a transport mechanism, transferring the tissue samples to the process chambers, the transport mechanism comprising a transport arm and a holder for several cassettes for tissue samples attached to a transport arm; and

a drive mechanism attached to the transport arm and causing a relative rotational motion between the transport mechanism and the baseplate,

wherein the rotational motion causes the transfer of the tissue samples between the process chambers;

wherein the process chambers are arranged around the axis of the rotational motion;

wherein the transport arm is lowered by gravity introducing the holder into the process chamber;

wherein at least one process chamber comprises a first opening in the floor; and

wherein the process chamber may be filled with a reagent or emptied of a reagent through the first opening simultaneously with the transferring.

2. The device according to claim 1, further comprising a lid attached to the top of the holder, wherein the lid closes the process chamber when the holder is lowered into the process chamber.

3. The device according to claim 2, wherein the lid has a freedom of movement, so that, when the holder is shaken in the process chamber by the transport mechanism, the lid remains seated on the process chamber.

4. The device according to claim 1, wherein at least one process chamber comprises a second opening, wherein the second opening in combination with the first opening allows continuous pumping of a reagent through the process chamber.

5. The device according to claim 1, wherein at least one process chamber has no openings in either its side wall or its floor and is heatable.

6. The device according to claim 1, wherein at least one process chamber has no openings in either its side wall or its floor and is capable of being manually filled with wax.

7. The device according to claim 1, wherein the process chambers are cup-shaped, and wherein at least one process chamber comprises the first opening and a second opening in the floor of the process chamber.

8. The device according to claim 7, wherein the volume of the process chambers is a multiple of the volume of a tissue sample contained in the holder.

9. The device according to claim 8, wherein the holder is manufactured out of plastic and is designed for single-use processing.

10. The device according to claim 1, further comprising several replaceable reservoirs for reagent positioned under the baseplate in the housing, wherein the reagent may be pumped from one of the reservoirs into one of the process chambers and from the process chamber back into that reservoir.

11. A method for processing of tissue samples in several process chambers, each of the process chambers comprising a side wall and a floor, comprising the following steps:

lowering a holder with cassettes for tissue samples into a loading position, comprising a first process chamber containing a reagent for intermediate storage of the tissue samples;

lifting of the holder by a transport arm attached to a transport mechanism;

transfer of the holder to a second process chamber;

filling simultaneously with the transfer the second process chamber through an opening in the floor of the second process chamber;

lowering of the holder into the second process chamber;

keeping the holder in the second process chamber for a time t₁;

removal of the holder from the second process chamber;

transfer of the holder to a third process chamber;

emptying simultaneously with the transfer the second process chamber;

filling simultaneously with the transfer the third process chamber;

lowering of the holder into the third process chamber;

keeping the holder in the third process chamber for a time t₂;

removal of the holder from the third process chamber;

emptying the third process chamber after the removal of the holder;

lowering of the holder into a first wax-filled process chamber;

keeping the holder in the wax-filled process chamber for a time t₃; and

lowering of the holder into an unloading station.

12. The method according to claim 11, wherein the time t₁ does not exceed 30 seconds and the second process chamber is filled with ethanol.

13. The method according to claim 11, wherein the time t₂ does not exceed 6 minutes and the third process chamber is filled with acetone.

14. The method according to claim 11, wherein the time t₃ does not exceed 6 minutes.

15. The method according to claim 14 further comprising a second wax-filled process chamber used after the first wax-filled process chamber to reduce transfer and contamination with a solvent.

16. The method according to claim 11, wherein the process chambers are filled and emptied from and into replaceable reservoirs in a lower part of a housing separated from an upper part of the housing by a baseplate.

17. The method according to claim 11, characterized wherein a relative movement is generated between the process chambers and a transport arm to transfer the holder from one process chamber to another process chamber.