Methods and systems for processing microscale devices for reuse

• US 7,514,046 B2
• Filed: 06/04/2004
• Issued: 04/07/2009
• Est. Priority Date: 10/31/2000
• Status: Active Grant

• Current Assignee: WAKO Pure Chemical Industries Limited (Fujifilm Holdings Corporation), Caliper Life Sciences Incorporated (Revvity, Inc.)
• Original Assignee: Caliper Life Sciences Incorporated (Revvity, Inc.)
• Inventors: Kechagia, Persefoni, Hayashi, Masayoshi, Donlon, Ed, Rulison, Aaron J., Brogden, Bruce, Greenstein, Michael
• Primary Examiner(s): Stinson; Frankie L
• Assistant Examiner(s): Waldbaum; Samuel A
• Application Number:
  US10/861,784
• Publication Number:
  US 20050019213A1
• Time in Patent Office: 1,768 Days
• Field of Search: 134/22.1, 134/26, 422/102
• US Class Current: 422/504
• CPC Class Codes: B01L 13/02 for receptacle or instruments
• CPC Class Codes: B01L 2300/022 Transponder chips
• CPC Class Codes: B01L 2300/024 Storing results with means integrated into the container
• CPC Class Codes: B01L 2400/0415 electrical forces, e.g. electrokinetic
• CPC Class Codes: B01L 2400/0487 fluid pressure, pneumatics
• CPC Class Codes: B01L 3/5027 by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip B01L3/5023 takes precedence; micromixers B01F33/30; microreactors for synthesis B01J19/0093; microcapillary devices in general B81B1/00
• CPC Class Codes: B01L 3/502715 characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
• CPC Class Codes: B01L 9/527 for microfluidic devices, e.g. used for lab-on-a-chip
• CPC Class Codes: G01N 1/40 Concentrating samples
• CPC Class Codes: G01N 27/44704 Details; Accessories
• CPC Class Codes: G01N 27/44773 Multi-stage electrophoresis, e.g. two-dimensional electrophoresis
• CPC Class Codes: G01N 27/44791 Microapparatus sample containers with integrated microfluidic structures B01L3/5027

Abstract

This invention provides methods and systems for flushing, washing, and priming microscale devices for reuse. Washing and priming methods include flowing solutions from a manifold to flush wells and microchannels of a microfluidic chip. Systems include manifolds adapted to seal and flow solutions or gasses into chip wells. Devices include microfluidic devices with data storage modules to track the reprocessing status of the microscale devices.

Claims

1. A system for processing a microscale device, the system comprising:

a manifold comprising a plurality of ring reservoirs in fluid contact with one or more mesoscale flush channels or one or more mesoscale waste channels, wherein two or more of the plurality of ring reservoirs are concentric and share a flowpath in one or more channel segments;

one or more orifices in fluid contact with the flush channels or waste channels, and adapted to functionally contact one or more wells of the microscale device; and

,

one or more process solutions in a flowpath comprising the one or more mesoscale channels, the one or more orifices, and the one or more wells of the microscale device.

2. The system of claim 1, wherein the microscale device comprises one or more microchannels in fluid contact with the one or more wells, and wherein the flowpath further comprises the one or more microchannels.

3. The system of claim 2, further comprising one or more control valves in the one or more waste channels that can direct the one or more process solutions into the one or more microchannels.

4. The system of claim 1, wherein the mesoscale channels comprise a dimension ranging from about 5 mm to about 0.1 mm.

5. The system of claim 1, wherein the flush channels and waste channels compromise one or more concentric segments.

6. The system of claim 1, wherein the flush channels or waste channels comprise a pressure differential.

7. The system of claim 1, wherein the one or more process solutions comprise:

NaOH, water, a reagent, a surfactant, a solvent, or a heated solution.

8. The system of claim 1, wherein the one or more process solutions comprise a wash solution comprising an alkaline solution of an ampholytic surfactant and chelator.

9. The system of claim 1, wherein the adapted orifices comprise:

o-rings, a tapered surface, or both.

10. The system of claim 1, wherein the wells comprise:

sample wells, reagent wells, waste wells, or a combination thereof.

11. The system of claim 1, wherein the orifices comprise one or more radial jets.

12. The system of claim 1, further comprising one or more reagent containers in fluid contact with the one or more ring reservoirs through solution supply ports.

13. The system of claim 1, wherein the manifold further comprises a multilayer structure.

14. The system of claim 1, wherein the manifold further comprises one or more control valves functionally associated with the flush channels or waste channels.

15. The system of claim 14, wherein one of the one or more control valves controls flow of the process solutions or a flushing gas to:

one of the one or more wells, two or more of the one or more wells, or one or more groups of wells.

16. The system of claim 15, wherein the groups of wells comprise:

sample wells, reagent wells, wash wells, or waste wells.

17. The system of claim 14, wherein the one or more control valves comprise:

pneumatic valves, solenoid valves, needle valves, sandwich valves, diaphragm valves, slider valves, or ball and seat valves.

18. The system of claim 1, further comprising an automated flow controller.

19. The system of claim 18, wherein the automated flow controller comprises:

a computer, an interface, solenoid valves, pneumatic valves, or an electronic pressure regulator.

20. The system of claim 1, further comprising a flushing gas in the flowpath.

21. The system of claim 1, further comprising a system configuration as an independent instrument or as a component of an analytical instrument.

22. The system of claim 1, further comprising a data storage module mounted to the microscale device.

23. The system of claim 1, wherein the process solution comprises a reagent to condition or prime the one or more wells or one or more microchannels.

24. A system for processing a microscale device, the system comprising:

a manifold comprising a plurality of ring reservoirs in fluid contact with one or more mesoscale flush channels or one or more mesoscale waste channels, wherein two or more of the plurality of ring reservoirs are concentric and share a flowpath in one or more channel segments;

one or more orifices in fluid contact with the flush channels or waste channels, and adapted to functionally contact one or more wells of the microscale device; and

,

a gas in a flowpath comprising the one or more mesoscale channels, the one or more orifices, and the one or more wells of the microscale device, wherein the flowpath comprises a pressure differential;

whereby process solutions or waste solutions are flushed from the manifold, wells, or microfluidic device.

25. The system of claim 24, wherein the contact between the one or more orifices and the one or more wells comprises a sealed contact.

26. The system of claim 24, wherein the waste channel comprises a pressure less than atmospheric pressure.

27. The system of claim 24, wherein the pressure differential ranges from about 1 psi to about 500 psi.

28. The system of claim 24, wherein the flowpath further comprises one or more microchannels.