Kits for detection of nucleic acids using invasive cleavage structures and flap endonucleases
First Claim
1. A kit for detecting a target sequence, comprising:
- i) nucleic acid molecules capable of forming an invasive cleavage structure in the presence of a target sequence; and
ii) an agent for detecting the presence of an invasive cleavage structure.
9 Assignments
0 Petitions
Accused Products
Abstract
The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. The structure-specific nuclease activity of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof. The present invention further relates to methods and devices for the separation of nucleic acid molecules based on charge. The present invention also provides methods for the detection of non-target cleavage products via the formation of a complete and activated protein binding region. The invention further provides sensitive and specific methods for the detection of nucleic acid from various viruses in a sample.
-
Citations
22 Claims
-
1. A kit for detecting a target sequence, comprising:
-
i) nucleic acid molecules capable of forming an invasive cleavage structure in the presence of a target sequence; and ii) an agent for detecting the presence of an invasive cleavage structure. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19)
-
-
20. A kit for detecting a target sequence, comprising:
-
a) nucleic acid molecules configured to form cleavage structure on a target sequence that is cleavable by a Mja FEN-1 endonuclease,wherein said cleavage structure comprises; i) said target sequence; ii) said nucleic acid molecules hybridized to said target nucleic acid to form a displaced 5′
nucleic acid; andb) a thermostable FEN-1 endonuclease for detecting the presence of a cleavage structure comprising a displaced 5′
arm. - View Dependent Claims (21, 22)
-
Specification