Zinc finger transcription factor differentiation proteins
First Claim
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1. An isolated transcription factor that comprises a first, second and third zinc finger domain in this order, wherein presence of the transcription factor in a vertebrate cell can alter the differentiation state of the cell, the transcription factor further comprises an activation domain, and the DNA contacting residues of the first, second, and third domains are as shown in reference sequence SEQ ID NO:
- 2 as follows;
(i) glutamine at position −
1 of the first zinc finger domain;
serine at position 2 of the first zinc finger domain;
asparagine at position 3 of the first zinc finger domain; and
arginine at position 6 of the first zinc finger domain;
(ii) glutamine at position −
1 of the second zinc finger domain;
serine at position 2 of the second zinc finger domain;
asparagine at position 3 of the second zinc finger domain; and
lysine at position 6 of the second zinc finger domain; and
(iii) cysteine at position −
1 of the third zinc finger domain;
serine at position 2 of the third zinc finger domain;
asparagine at position 3 of the third zinc finger domain; and
arginine at position 6 of the third zinc finger domain.
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Abstract
The disclosure describes artificial chimeric transcription factors that include at least one zinc finger domain and that can regulate cellular differentiation, for example, a neuronal cell phenotype or an osteoblast phenotype.
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Citations
15 Claims
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1. An isolated transcription factor that comprises a first, second and third zinc finger domain in this order, wherein presence of the transcription factor in a vertebrate cell can alter the differentiation state of the cell, the transcription factor further comprises an activation domain, and the DNA contacting residues of the first, second, and third domains are as shown in reference sequence SEQ ID NO:
- 2 as follows;
(i) glutamine at position −
1 of the first zinc finger domain;
serine at position 2 of the first zinc finger domain;
asparagine at position 3 of the first zinc finger domain; and
arginine at position 6 of the first zinc finger domain;(ii) glutamine at position −
1 of the second zinc finger domain;
serine at position 2 of the second zinc finger domain;
asparagine at position 3 of the second zinc finger domain; and
lysine at position 6 of the second zinc finger domain; and(iii) cysteine at position −
1 of the third zinc finger domain;
serine at position 2 of the third zinc finger domain;
asparagine at position 3 of the third zinc finger domain; and
arginine at position 6 of the third zinc finger domain. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13)
- 2 as follows;
- 14. An isolated transcription factor that comprises amino acids 31 to 109 of SEQ ID NO:
Specification