Immunoassay probe

• US 7,514,270 B2
• Filed: 04/10/2003
• Issued: 04/07/2009
• Est. Priority Date: 04/12/2002
• Status: Expired due to Fees

• Current Assignee: Instrumentation Lab Co (Instrumentation Lab)
• Original Assignee: Instrumentation Lab Co (Instrumentation Lab)
• Inventors: Achter, Eugene K., Trotta, Stephen S., Schroeder, Hartmut Richard
• Primary Examiner(s): Shibuya; Mark L
• Assistant Examiner(s): Do; Pensee T
• Application Number:
  US10/411,441
• Publication Number:
  US 20030194799A1
• Time in Patent Office: 2,189 Days
• Field of Search: 436/526, 436/47, 436/807, 209/214, 210/222, 210/695, 422/65, 422/68.1
• US Class Current: 436/526
• CPC Class Codes: B03C 1/288 disposed at the outer circumference of a recipient
• CPC Class Codes: B03C 2201/26 for use in medical applications
• CPC Class Codes: G01N 35/0098 involving analyte bound to insoluble magnetic carrier, e.g. using magnetic separation magnetic particles used in immunoassays G01N33/54326; magnetic separation in general B03C
• CPC Class Codes: Y10S 436/807 Apparatus included in process claim, e.g. physical support structures
• CPC Class Codes: Y10T 436/113332 with conveyance of sample along a test line in a container or rack

Abstract

An analytical instrument for the immobilization of antigens to be detected in an immunoassay using mangetizable beads. The instrument includes a probe containing magnetizable particles and a plurality of magnetic devices for applying a magnetic field gradient to the particles in the probe reservoir. The probe is movable between the plurality of magnetic devices and at least one of the probe positions may be used to immobilize the magnetic particles in the fluid medium and at least another position may be used to resuspend the particles in a fluid medium.

Claims

1. A device for manipulating a magnetizable particle suspended in a solution, comprising:

a first magnet;

a second magnet; and

a probe for holding and transporting said magnetizable particle comprising an open top and an open bottom, wherein said probe is transportable through a plurality of positions between said first magnet and said second magnet in a horizontal direction for manipulating said magnetizable particle, wherein said magnetizable particle is resuspended in one of said probe positions, and wherein the strength of said first magnet is weaker than the strength of said second magnet.

2. The device according to claim 1 further comprising a dilutor assembly, wherein said dilutor assembly introduces a fluid into the open top of the probe, and wherein the open top of the probe is opposite to the open bottom of said probe.

3. The device according to claim 1 wherein said probe further comprises a thermal control element.

4. The device according to claim 1 wherein said probe further comprises a liquid level sensing element.