Multisignal labeling reagents, and processes and uses therefor
First Claim
1. A composition of matter comprising a nucleic acid strand or a complex of two or more nucleic acid strand wherein said strand or complex comprises one or more reactive groups and two or more labeled nucleotides or labeled nucleotide analogs, wherein said reactive group is isothiocyanate, isocyanate, monochlorotriazine, dichlorotriazine, mono- or di-halogen substituted pyridine, mono- or di-halogen substituted diazine, maleimide, aziridine, sulfonyl halogen substituted diazine, maleimide, aziridine, sulfonyl halide, acid halide, hydroxysuccinimide ester, hydroxysulfosuccinimide ester, imido ester, hydrazine, azidonitrophenyl, azide, 3-(2-pyridyl dithio)-proprionamide, glyoxal, aldehyde, carbon-carbon double bonds, mercury salts, and/or any group capable of reacting with carbon-carbon double bonds, amines, hydroxyl groups, sulfhydryl groups and/or halogens.
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Abstract
The present invention provides multisignal labeling reagents and these are useful in a number of biochemical applications, including the manufacture of biomolecular probes and their use in detecting or amplifying analyte-specific moieties.
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Citations
10 Claims
- 1. A composition of matter comprising a nucleic acid strand or a complex of two or more nucleic acid strand wherein said strand or complex comprises one or more reactive groups and two or more labeled nucleotides or labeled nucleotide analogs, wherein said reactive group is isothiocyanate, isocyanate, monochlorotriazine, dichlorotriazine, mono- or di-halogen substituted pyridine, mono- or di-halogen substituted diazine, maleimide, aziridine, sulfonyl halogen substituted diazine, maleimide, aziridine, sulfonyl halide, acid halide, hydroxysuccinimide ester, hydroxysulfosuccinimide ester, imido ester, hydrazine, azidonitrophenyl, azide, 3-(2-pyridyl dithio)-proprionamide, glyoxal, aldehyde, carbon-carbon double bonds, mercury salts, and/or any group capable of reacting with carbon-carbon double bonds, amines, hydroxyl groups, sulfhydryl groups and/or halogens.
Specification