Multidentate arrays

• US 7,517,979 B2
• Filed: 06/24/2004
• Issued: 04/14/2009
• Est. Priority Date: 07/01/1999
• Status: Expired due to Fees

• Current Assignee: Agilent Technologies, Inc.
• Original Assignee: Agilent Technologies, Inc.
• Inventors: Wolber, Paul K.
• Primary Examiner(s): Horlick; Kenneth R.
• Assistant Examiner(s): Thomas; David C
• Application Number:
  US10/877,342
• Publication Number:
  US 20050095614A1
• Time in Patent Office: 1,755 Days
• Field of Search: None
• US Class Current: 536/25.3
• CPC Class Codes: C12Q 1/6837 using probe arrays or probe chips C12Q1/6874 takes precedence
• CPC Class Codes: C12Q 2525/161 incorporating target specific and non-target specific sites
• CPC Class Codes: C12Q 2565/107 Alteration in the property of hybridised versus free label oligonucleotides
• CPC Class Codes: C12Q 2565/513 characterised by the pattern of the arrayed oligonucleotides

Abstract

A method of evaluating for the presence of a target polynucleotide in a sample, using an addressable array of multiple polynucleotide probes linked to a substrate. The sample is exposed to the array and a set of polynucleotide target probes, such that target polynucleotide which may be present will bind to a predetermined feature of the array through multiple target probes of the set by forming at respective target regions on a target molecule, simultaneous hybrids with anti-target regions of the multiple target probes. A binding pattern on the array is observed and the presence of the target polynucleotide evaluated based on the observed binding pattern. Kits using such arrays, and methods for selecting target probes are further provided.

Claims

1. A method for producing an addressable array of features comprising:

receiving a target polynucleotide;

determining an evaluated probe set for said target polynucleotide, wherein said set comprises probes that simultaneously bind to said target when said probes are present in the same feature of an addressable array; and

fabricating an addressable array comprising said evaluated probe set present in the same feature of said addressable array as a mixed probe feature.

2. The method according to claim 1, wherein said method comprises employing a computer having a display means and an input means.

3. The method according to claim 1, wherein said determining comprises accepting or rejecting candidate probes for said evaluated probe set.

4. The method according to claim 1, wherein said array is useful in diagnostic, screening or gene expression applications.

5. The method according to claim 1, wherein said array is useful in diagnostic applications.

6. The method according to claim 1, wherein said array comprises a synthesized or spotted array.

7. The method according to claim 1, wherein said method comprises communicating information between remote locations.

8. The method according to claim 1, wherein said receiving comprises receiving a sequence of said target polynucleotide.

9. A method for providing custom addressable arrays of features, comprising the acts of:

receiving a user selection of one or more probe set identifiers that each identify a plurality of potential probes;

determining verified probe sets of verified probes corresponding to the probe set identifiers, wherein said sets comprise probes that simultaneously bind to a target when said probes are present in the same feature of an addressable array;

generating a custom addressable array design based, at least in part, upon the verified probe sets; and

providing to the user one or more addressable arrays based on the addressable array design, wherein said probes that simultaneously bind to a target are present in the same feature of said addressable array as a mixed probe feature.

10. A method for providing custom addressable arrays of features, comprising the acts of:

receiving a user selection of one or more probe set identifiers that identify one or more potential probes;

determining verified probe sets of verified probes corresponding to the probe set identifiers, wherein said sets comprise probes that simultaneously bind to a target when said probes are present in the same feature of said addressable array;

generating a custom addressable array design based, at least in part, upon the verified probe sets wherein said probes that simultaneously bind to a target are present in the same feature of said addressable array as a mixed probe feature; and

enabling for display to the user a representation of one or more aspects of the custom probe array design via one or more graphical user interfaces enabled to receive a user selection specifying acceptance, modification, or rejection of the custom probe array design; and

providing to the user one or more probe arrays based on the probe array design and responsive to the user specification of acceptance or modification.

11. The method of claim 10, wherein-one or more of the probe arrays is constructed and arranged to diagnose a disease or medical condition.

12. The method of claim 10, wherein:

the probe array comprises a synthesized or spotted array.

13. The method of claim 10, wherein:

the user selection is received over the Internet.

14. The method of claim 10, wherein:

the probe set identifiers comprise sequence information.