Image acquisition, processing, and display
First Claim
Patent Images
1. An apparatus for imaging, comprising:
- a light source emitting a polarized light beam;
an optical assembly including a light reflection surface, wherein the light beam from the light source is reflected by the light reflection surface to provide an evanescent field adjacent the light reflection surface, the light reflection surface being adapted to allow placing thereon a specimen array such that the specimen array in the evanescent field causes spatially distributed polarization changes in the cross-section of the light beam;
a two-dimensional array detector positioned to detect the spatially distributed polarization changes caused by the specimen array; and
a processor operably coupled to the two-dimensional array detector, the processor processing data from the two-dimensional array detector to provide a two-dimensional representation of the spatially distributed polarization changes occurring in the specimen array in real-time.
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Abstract
Image data is acquired, processed, and/or displayed in accordance with an embodiment of the present disclosure to display, monitor, and/or demonstrate the progress of an experiment substantially in real-time and with high sensitivity. In one embodiment, at least one time-resolved value of spatially distributed polarization change data is provided and displayed. Advantageously, real-time processing and display of data is provided such that discussion and collaboration about the experiment may occur, time-resolved data is not lost, and resources are not wasted.
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Citations
15 Claims
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1. An apparatus for imaging, comprising:
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a light source emitting a polarized light beam; an optical assembly including a light reflection surface, wherein the light beam from the light source is reflected by the light reflection surface to provide an evanescent field adjacent the light reflection surface, the light reflection surface being adapted to allow placing thereon a specimen array such that the specimen array in the evanescent field causes spatially distributed polarization changes in the cross-section of the light beam; a two-dimensional array detector positioned to detect the spatially distributed polarization changes caused by the specimen array; and a processor operably coupled to the two-dimensional array detector, the processor processing data from the two-dimensional array detector to provide a two-dimensional representation of the spatially distributed polarization changes occurring in the specimen array in real-time. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 15)
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9. A method of imaging, comprising:
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passing a polarized light beam into an optical assembly including a control layer and a light reflection surface to provide an evanescent field with controlled height and intensity adjacent the light reflection surface, a specimen array in the evanescent field causing spatially distributed polarization changes in the cross-section of the light beam; passing the reflected light beam out of the optical structure; detecting the spatially distributed polarization changes caused by the specimen array; and processing the detected spatially distributed polarization changes to provide a two-dimensional representation of the spatially distributed polarization changes occurring in the specimen array in real-time. - View Dependent Claims (10, 11, 12, 13, 14)
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Specification