Prism design for scanning applications and illumination of microscopy sample
First Claim
Patent Images
1. A scanning apparatus comprising:
- a light source for generating a light beam; and
an angular prism coupled to a sample at a base interface;
characterised in that the base angle θ
p of the prism satisfies the equations;
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Abstract
There is disclosed a prism for use in scanning applications such as total internal reflection microscopy in which the prism is translated relative to an incident light beam. A geometry is disclosed which cancels walk of the beam footprint at the base of the prism. Walk of the beam footprint due to irregularities in a largely planar sample surface located at the prism base are cancelled by coupling movement of the incident light beam to movement of the sample in the field of view of an objective lens, for example as part of an autofocus arrangement.
22 Citations
25 Claims
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1. A scanning apparatus comprising:
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a light source for generating a light beam; and an angular prism coupled to a sample at a base interface; characterised in that the base angle θ
p of the prism satisfies the equations; - View Dependent Claims (2, 3, 4)
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5. A scanning method comprising the steps of:
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generating a light beam; providing an angular prism in the path of the light beam, the prism being coupled to a sample at a base interface; and moving the prism and sample relative to the light beam; characterised in that the base angle θ
p of the prism satisfies the equations; - View Dependent Claims (6)
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7. Apparatus for imaging a sample, comprising:
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an objective lens having a focal plane and an optical axis, the lens being arranged to collect light from a region of interest defined relative to said optical axis; an illumination director controllable to direct a beam of light along an optical path so as to illuminate the sample in the region of interest at an angle oblique to said optical axis; and an illumination director controller arranged to control the illumination director relative to the sample such that the optical path remains directed to the sample in the region of interest following replacement of the sample, or movement of the sample relative to the objective lens, said apparatus further comprising an angular prism having a base and a side face having a base/side face angle θ
p therebetween, the sample being adjacent to the base of the prism, the beam of light being directed through the side face of the prism at an angle of incidence θ
i so as to illuminate the sample at the base at a coupling angle θ
c. - View Dependent Claims (8, 9)
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10. Apparatus for imaging a sample, comprising:
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an objective lens having a focal plane and an optical axis, the lens being arranged to collect light from a region of interest defined relative to said optical axis; an illumination director controllable to direct a beam of light along an optical path so as to illuminate the sample in the region of interest at an angle oblique to said optical axis; and an illumination director controller arranged to control the illumination director relative to the sample such that the optical path remains directed to the sample in the region of interest following replacement of the sample, or movement of the sample relative to the objective lens, wherein the illumination director controller is arranged to adjust the illumination director synchronously with movements parallel to the optical axis of the sample within the region of interest. - View Dependent Claims (11)
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12. Apparatus for imaging a sample, comprising:
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an objective lens having a focal plane and an optical axis, the lens being arranged to collect light from a region of interest defined relative to said optical axis; an illumination director controllable to direct a beam of light along an optical path so as to illuminate the sample in the region of interest at an angle oblique to said optical axis; and an illumination director controller arranged to control the illumination director relative to the sample such that the optical path remains directed to the sample in the region of interest following replacement of the sample. or movement of the sample relative to the objective lens, wherein the illumination director controller is arranged to adjust the illumination director synchronously with movements of the focal plane in a direction parallel to the optical axis and relative to the sample such that the optical path remains directed to the intersection of said region of interest and said focal plane.
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13. Apparatus for imaging a sample, comprising:
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an objective lens having a focal plane and an optical axis, the lens being arranged to collect light from a region of interest defined relative to said optical axis; an illumination director controllable to direct a beam of light along an optical path so as to illuminate the sample in the region of interest at an angle oblique to said optical axis; and an illumination director controller arranged to control the illumination director relative to the sample such that the optical path remains directed to the sample in the region of interest following replacement of the sample, or movement of the sample relative to the objective lens, said apparatus further comprising an autofocus arranged such that the focal plane of the objective lens tracks the sample in the region of interest. - View Dependent Claims (14, 15, 16)
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17. Apparatus for imaging a sample, comprising:
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an objective lens having a focal plane and an optical axis, the lens being arranged to collect light from a region of interest defined relative to said optical axis; an illumination director controllable to direct a beam of light along an optical path so as to illuminate the sample in the region of interest at an angle oblique to said optical axis; and an illumination director controller arranged to control the illumination director relative to the sample such that the optical path remains directed to the sample in the region of interest following replacement of the sample. or movement of the sample relative to the objective lens, wherein at least a part of the objective lens is moveable along the optical axis so as to bring a part of the sample which is within the region of interest into focus, and the illumination director controller comprises a coupling arranged to adjust the illumination director synchronously with the movement of the at least part of the objective along the optical axis. - View Dependent Claims (18)
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19. Apparatus for imaging a sample, comprising:
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an objective lens having a focal plane and an optical axis, the lens being arranged to collect light from a region of interest defined relative to said optical axis; an illumination director controllable to direct a beam of light along an optical path so as to illuminate the sample in the region of interest at an angle oblique to said optical axis; and an illumination director controller arranged to control the illumination director relative to the sample such that the optical path remains directed to the sample in the region of interest following replacement of the sample, or movement of the sample relative to the objective lens, wherein the illumination director controller comprises a direct mechanical coupling.
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20. Apparatus for imaging a sample, comprising:
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an objective lens having a focal plane and an optical axis, the lens being arranged to collect light from a region of interest defined relative to said optical axis; an illumination director controllable to direct a beam of light along an optical path so as to illuminate the sample in the region of interest at an angle oblique to said optical axis; and an illumination director controller arranged to control the illumination director relative to the sample such that the optical path remains directed to the sample in the region of interest following replacement of the sample, or movement of the sample relative to the objective lens, wherein the illumination director controller comprises an electrical coupling.
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21. Apparatus for imaging a sample, comprising:
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an objective lens having a focal plane and an optical axis, the lens being arranged to collect light from a region of interest defined relative to said optical axis; an illumination director controllable to direct a beam of light along an optical path so as to illuminate the sample in the region of interest at an angle oblique to said optical axis; and an illumination director controller arranged to control the illumination director relative to the sample such that the optical path remains directed to the sample in the region of interest following replacement of the sample, or movement of the sample relative to the objective lens, wherein the objective lens is positioned on a first side of the sample, and the illumination director is positioned on the opposing side of the sample.
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22. Apparatus for imaging a sample, comprising:
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an objective lens having a focal plane and an optical axis, the lens being arranged to collect light from a region of interest defined relative to said optical axis; an illumination director controllable to direct a beam of light along an optical path so as to illuminate the sample in the region of interest at an angle oblique to said optical axis; and an illumination director controller arranged to control the illumination director relative to the sample such that the optical path remains directed to the sample in the region of interest following replacement of the sample, or movement of the sample relative to the objective lens, wherein the apparatus is a total internal reflection fluorescence microscope. - View Dependent Claims (23)
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24. Apparatus for imaging a sample, comprising:
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an objective lens having a focal plane and an optical axis, the lens being arranged to collect light from a region of interest defined relative to said optical axis; an illumination director controllable to direct a beam of light along an optical path so as to illuminate the sample in the region of interest at an angle oblique to said optical axis; and an illumination director controller arranged to control the illumination director relative to the sample such that the optical path remains directed to the sample in the region of interest following replacement of the sample, or movement of the sample relative to the objective lens, wherein the illumination director is a first mirror. - View Dependent Claims (25)
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Specification