Method for elucidating reaction dynamics of photoreactive compounds from optical signals affected by an external magnetic field

• US 7,519,411 B2
• Filed: 09/26/2003
• Issued: 04/14/2009
• Est. Priority Date: 09/26/2003
• Status: Active Grant

• Current Assignee: Institut national d'optique
• Original Assignee: Institut national d'optique
• Inventors: Long, William
• Primary Examiner(s): Casler; Brian
• Assistant Examiner(s): KISH, JAMES M
• Application Number:
  US10/671,704
• Publication Number:
  US 20050069497A1
• Time in Patent Office: 2,027 Days
• Field of Search: 600/407, 600/473, 600/475, 600476-478
• US Class Current: 600/407
• CPC Class Codes: A61B 5/0059 using light, e.g. diagnosis by transillumination, diascopy, fluorescence A61B5/0093, A61B5/1455, A61B5/02416 and A61B5/0261 take precedence
• CPC Class Codes: A61B 5/14551 for measuring blood gases
• CPC Class Codes: A61N 2/002 in combination with another treatment
• CPC Class Codes: A61N 5/062 Photodynamic therapy, i.e. excitation of an agent

Abstract

A method elucidates reaction dynamics of photoreactive compounds from time-resolved optical signals affected by an external magnetic field. A coil or magnet applies a magnetic field at a region of interest (ROI). A light or laser illuminates the ROI with a probe beam. An optical fiber collects light emitted by the probe beam; and a computer analyses the collected light.

Claims

1. A method for elucidating reaction dynamics of photoreactive compounds in vivo, comprising the steps of:

a) turning on a probe beam;

b) irradiating tissue within a region of interest (ROI) with the probe beam;

c) recording fluorescence, phosphorescence and absorption characteristics of the tissue at at least one wavelength;

d) administering a photoreactive agent to the ROI;

e) irradiating the ROI with the probe beam;

f) recording luminescence intensity and lifetime and absorption characteristics of the ROI at at least one wavelength when B =0 Tesla;

g) applying a magnetic field of a predetermined strength to the ROI so that the combination of the magnetic field and irradiation induces a chemical reaction in said photoreactive agent;

h) simultaneously recording the luminescence intensity and lifetime and absorption characteristics of the ROI at at least one wavelength when B>

0 Tesla;

i) repeating steps g) and h) for different magnetic field strengths;

j) turning off said probe beam;

k) processing the recorded data obtained in steps c), f) and h); and

l) displaying the results obtained by said processing on a display.

2. The method of claim 1, wherein a therapeutic beam is employed, and wherein said method includes the step of turning on the therapeutic beam for a set time then turning it off, then repeating steps a) and e)through j).

3. The method of claim 2, wherein the probe beam and the therapeutic beam are of the same wavelength and are generated from the same source.

4. The method of claim 2, wherein the probe beam and the therapeutic beam are of different wavelengths.

5. The method of claim 2, wherein the probe beam and the therapeutic beam use the same source and wavelength, and where the therapeutic beam is more intense than the probe beam.

6. The method of claim 1, wherein said recording and photodynamic therapy (PDT) are simultaneous.

7. The method of claim 1, wherein multiple wavelengths are measured at the same time.

8. The method of claim 1, wherein said method further comprises the step of recording tissue oxygenation and blood perfusion of the ROI at least one of before administering the photoreactive agent and after turning off the probe beam.