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PCR compatible nucleic acid sieving matrix

  • US 7,521,186 B2
  • Filed: 09/10/2003
  • Issued: 04/21/2009
  • Est. Priority Date: 03/20/2000
  • Status: Expired due to Fees
First Claim
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1. A method of performing PCR and separating one or more PCR products, the method comprising:

  • (i) mixing one or more PCR reaction components with an unpolymerized sieving medium in a channel of a microfluidic device to provide an unpolymerized PCR sieving medium within the channel;

    (ii) thermocycling the PCR sieving medium to produce one or more PCR products;

    (iii) polymerizing the sieving medium after thermocycling is completed, wherein the polymerized sieving medium has a polymer concentration that is less than 0.4%; and

    (iv) separating the one or more PCR products by flowing the one or more PCR products through the polymerized sieving medium.

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