Devices for generating detectable polymers

• US 7,521,189 B2
• Filed: 10/12/2006
• Issued: 04/21/2009
• Est. Priority Date: 10/12/2006
• Status: Active Grant

• Current Assignee: Fair Isaac Corporation
• Original Assignee: Fair Isaac Corporation
• Inventors: Engelhard, Eric K.
• Primary Examiner(s): Horlick; Kenneth R.
• Assistant Examiner(s): Baughman; Molly E
• Application Number:
  US11/549,008
• Publication Number:
  US 20080090232A1
• Time in Patent Office: 922 Days
• Field of Search: None
• US Class Current: 435/6.16
• CPC Class Codes: B01J 2219/00317 Microwell devices, i.e. having large numbers of wells
• CPC Class Codes: B01J 2219/00576 fluorophore
• CPC Class Codes: B01J 2219/00722 Nucleotides
• CPC Class Codes: B01L 2300/0816 Cards, e.g. flat sample carriers usually with flow in two horizontal directions
• CPC Class Codes: B01L 2300/0864 comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
• CPC Class Codes: B01L 3/5025 for parallel transport of multiple samples
• CPC Class Codes: B01L 3/5027 by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip B01L3/5023 takes precedence; micromixers B01F33/30; microreactors for synthesis B01J19/0093; microcapillary devices in general B81B1/00
• CPC Class Codes: B01L 7/52 with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples amplification or hybridisation processes per se C12Q1/68; controlling sequential reactions for synthesis B01J19/0046
• CPC Class Codes: C12Q 1/701 Specific hybridization probes

Abstract

This document provides systems, devices, and methods involved in generating detectable polymers. For example, diagnostic systems, diagnostic devices, primer systems, and collections of primer systems are provided.

Claims

1. A device comprising a housing having a plurality of locations, wherein at least one of said locations comprises any one of Primer Systems 1, 2, or 3 of Table 1.

2. The device of claim 1, wherein each of said locations is a chamber.

3. The device of claim 1, wherein each of said locations is a well.

4. The device of claim 1, wherein at least one of said locations comprises a different primer pair.

5. The device of claim 4, wherein said different primer pair capable of producing an amplification product from human nucleic acid.

6. The device of claim 1, wherein each of said locations comprises an intercalating dye.

7. The device of claim 6, wherein said intercalating dye is a green fluorescent dye.

8. The device of claim 6, wherein said intercalating dye is SYBR Green, LC Green, or SYTO9.

9. A method for detecting a respiratory syncytial virus within a sample, wherein said method comprises:

(a) performing a nucleic acid amplification reaction using said sample as a source of template and a diagnostic device, wherein said device comprises a housing having a plurality of locations, wherein at least one of said locations contains any one of Primer Systems 1, 2, or 3 of Table 1, and

(b) determining whether or not the location containing said primer system comprises said amplification product, thereby detecting a respiratory syncytial virus.

10. The method of claim 9, wherein said sample is a mucus sample obtained from a human.

11. The method of claim 9, wherein each of said locations comprises an intercalating dye, wherein each amplification product, when produced, is labeled with said intercalating dye, and wherein said determining step (b) comprises a signal from said dye.

12. The method of claim 9, wherein said amplification reaction is performed in a thermal cycler device configured to receive said diagnostic device.

13. The method of claim 9, wherein said determining step (b) is performed using a dye reader device configured to receive said diagnostic device.

14. The method of claim 9, wherein said amplification reaction and said determining step (b) are performed in a machine configured to receive said diagnostic device, said machine comprising a thermal cycler device and a dye reader device.

15. The method of claim 14, wherein said machine is capable of providing output indicating the presence of said respiratory syncytial virus.

16. The method of claim 14, wherein said machine is capable of providing output identifying indicating the primer system that detected the presence of said respiratory syncytial virus.

17. The method of claim 16, wherein said output is a paper printout or a computer readable file.