Cell lysis by heating-cooling process through endothermic reaction

• US 7,521,246 B2
• Filed: 01/19/2006
• Issued: 04/21/2009
• Est. Priority Date: 01/20/2005
• Status: Active Grant

• Current Assignee: Samsung Electronics Co. Ltd.
• Original Assignee: Samsung Electronics Co. Ltd.
• Inventors: Yang, Ji-yeon, Cho, Yoon-kyoung, Kim, Jin-tae, Kim, Sook-young, Lee, Young-sun
• Primary Examiner(s): WALLENHORST, MAUREEN
• Application Number:
  US11/335,414
• Publication Number:
  US 20060258012A1
• Time in Patent Office: 1,188 Days
• Field of Search: 436/63, 436/147, 436/174, 436/177, 422/68.1, 422/82.12, 422/61, 422/101, 435/4, 435/29
• US Class Current: 436/63
• CPC Class Codes: C12M 47/06 Hydrolysis; Cell lysis; Extraction of intracellular or cell wall material lysis of microorganisms C12N1/06; extracting or separating nucleic acids from biological samples C12N15/1003
• CPC Class Codes: C12M 47/20 Heating or cooling heating or cooling apparatus for laboratory uses B01L7/00
• CPC Class Codes: C12N 1/066 by physical methods
• CPC Class Codes: Y10T 436/25 including sample preparation

Abstract

Provided is a cell lysis method including: preparing a cell sample to be lysed; heating the cell sample; and cooling the cell sample by causing an endothermic reaction near the cell sample. According to the method, cell lysis can be simply and conveniently performed without regard to location and without additional devices since a separate energy source is not required and the apparatus is portable. In particular, when cell lysis is performed in a biochip using a small amount of sample, a greater cell lysis effect can be obtained. In addition, cell lysis efficiency is significantly improved, compared to when only heating is performed.

Claims

1. A cell lysis method comprising:

heating a cell sample to be lysed; and

cooling the cell sample by causing an endothermic reaction near the cell sample;

wherein heating and cooling are repeated at least twice; and

wherein heating and cooling result in lysis of the cells in the cell sample.

2. The cell lysis method of claim 1, wherein the heating is performed using heat generated during an exothermic reaction.

3. The cell lysis method of claim 1, wherein the heating is performed at a temperature of 90°

C. or higher and 100°

C. or lower, and the cooling is performed at a temperature of 30°

C. or lower and −

30°

C. or higher.

4. The cell lysis method of claim 1, wherein the endothermic reaction is a reaction between NH₄NO₃ (s) and H₂O(l) or a reaction of Ba(OH)₂8H₂O (s) and NH₄NO₃ (s).

5. A cell lysis apparatus comprising:

a heating portion, wherein the heating portion contains a solid material consisting of 5 to 7 wt % of MgCl₂ or MgSO₄ and 5 to 7 wt % to of NaOH with respect to 100 wt % of CaO to induce an exothermic reaction;

a cooling portion; and

a sample containing channel which alternately passes through the heating portion and the cooling portion at least one time.

6. The cell lysis apparatus of claim 5, wherein the cooling portion contains at least two materials capable of inducing an endothermic reaction by being mixed together.

7. The cell lysis apparatus of claim 6, wherein the at least two materials include NH₄NO₃ or Ba(OH)₂8H₂O.

8. The cell lysis apparatus of claim 5, wherein the sample containing channel has a sample inlet and a sample outlet.

9. The cell lysis apparatus of claim 8, wherein the sample outlet is connected to a PCR chamber.

10. The cell lysis apparatus of claim 5, being in the form of a portable package.